EGFR基因单核苷酸多态性与胃癌的相关性

Correlation between the EGFR gene single nucleotide polymorphisms and gastric cancer

目的探讨表皮生长因子受体(EGFR)rs 28384375C/T单核苷酸多态性在胃癌发生、发展中的作用。方法采用PCR-限制性片断长度多态性(PCR-RFLP)法对61例胃癌患者(胃癌组)和20例健康查体正常者(对照组)的EGFR基因单核苷酸多态性进行检测,并分析其与幽门螺杆菌(HP)感染的相关性。结果①EGFR位点:胃癌组EGFR基因型频率分别为C/T26.22%、T/T73.78%;对照组分别为C/T5.00%、T/T95.00%,两组均未发现C/C基因型;P=0.031 6;②C、T等位基因频率:胃癌组分别为13.11%、86.89%,对照组分别为2.50%、97.50%,P=0.044 3;③胃癌组两种基因型者HP感染检出率无统计学意义;④EGFR C/T多态性与胃癌淋巴结转移、远处转移、TNM分期和组织分化程度相关(P<0.05);与患者性别、年龄、胃癌发生部位、浸润深度、原发肿瘤侵犯范围和组织类型无相关性。结论EGFR单核苷酸多态性可促进胃癌的发生发展;与HP感染无明显相关性。

Objective To explore the role of epidermal growth factor receptor （EGFR） rs28384375C/T single nueleo- tide polymorphisms in the occurrence and development of gastric cancer. Methods A total of 61 eases of gastric carcinoma （gastric carcinoma group） and 20 healthy controls （control group）were genotyped by PCR-restrietion fragment length poly- morphism （PCR-RFLP） method, and the correlation between which and Helieobaeter pylori （HP） infection was analyzed. Results ①EGFR loci ：The frequencies of C/T and T/T genotypes of EGFR rs 28384375 C/T were 26.22% ,73.78% in gastric carcinoma group and 5.00% ,95.00% in control group, P =0.0316. C./C genotype was not found in both groups. ②frequencies of the allele C and T： Which were 13.11% ,86.89% in gastric carcinoma group and 2.50% ,97.50% re- spectively in control group,P =0. 0443.③ There was no significant difference of HP infection relevance ratio between the EGFRrs 28384375 C/T gene in gastric cancer group. ④The polymorphisms of EGFRrs 28384375 C/T were correlated to lymph node metastasis ,distant metastasis , clinical stage and the degree of differentiation （P 〈 0.05 ） , but not correlated with sex,age,the site of tumor, the depth of invasion,the extent of primary tumor invasion and histological types. Conclusion The polymorphisms of EGFRrs 28384375 C / T can promote the occurrence and development of gastric cancer, and which is not correlated with HP infection.