E6相关蛋白与前列腺癌细胞迁移浸润的实验研究

The empirical study on E6-AP with carcinoma of prostate cell migration and infiltration

目的:研究E6相关蛋白与前列腺癌细胞迁移浸润的相关性,为探讨前列腺癌的浸润转移机制提供实验依据。方法:应用电穿孔方法将线性化的PrevTet-off-in调控质粒和PrevTRE2-flag-E6AP重组质粒导入前列腺癌细胞(Lncap),建立稳定表达E6-AP的前列腺癌细胞。应用MTT实验检测细胞的增殖;应用Matrigel Invasion Chamber检测肿瘤细胞迁移浸润特性。结果:MTT结果显示,稳定转染表达E6-AP的前列腺癌细胞比未转染的细胞具有较强的增殖活性;在细胞的迁移浸润试验中,表达E6-AP的稳定转染细胞具有较强的爬行与附着能力。结论:E6相关蛋白与前列腺癌细胞迁移浸润性密切相关。

Objeetive： The carcinoma of prostate is a prostate gland familiar malignant tumor. It transfers gradually People pay attention to its migration and infiltration . This text mainly studies the relativity that the E6 - AP with carcinoma of prostate cell migration and infiltration . For inquirying into the gradually transfer of the carcinoma of prostate mechanism to provide an experiment. Methods： Apply the electricity boring method turns line of PrevTet - off- in,adjusting to control the quality grain and the PrevTRE2 - flag - E6 AP reorganization plasmid dueting prostate gland cancer cell（ Lneap）, and build up the prostate gland eancer of the stability expression E6 - AP. Apply the M TT experiment an examination cell to propagate. The applied Matrigel Invasion Chamber examination tumor cell moves gradually characteristic. Results： The MTT shows the stability turns the cell that the prostate gland cancer cell of dye the expression E6 - AP ratio didn＇t turn to dye to have as a result stronger propagate an activity. The stability that expresses E6 - AP turns to dye cell to have stronger crawl along and adhere to an ability. Conclusion： The E6 - AP albumen and carcinoma of prostate cell migration and infiltration is closely related