HIV-1暴露未感染人群DC-SIGN和DC-SIGNR基因多态性

Analysis of gene polymorphisms of DC-SIGN and SIGNR among HIV-1 exposures, seronegative exposers and healthy groups.

目的研究HIV-1高暴露未感染人群(ESN)、正常对照人群和HIV-1携带者DC-SIGN和DC-SIGNR基因型和基因频率分布,探讨这两个基因多态性与HIV-1不易感性的关系。方法应用PCR技术扩增两个基因颈区重复序列,以琼脂糖凝胶电泳区分两个基因不同重复序列的电泳图差异,以卡方检验分析基因型和基因频率分布差异。结果DC-SIGN在三组人群中以7/7型为主,三组人群共检出9个非7/7基因型,但DC-SIGN各基因型分布差异无统计学意义(P=0.648);DC-SIGNR具有高度多态性,7/5、5/5和6/7基因型的频率从ESN人群、健康对照人群和HIV-1携带者逐渐降低,同时5和6等位基因的频率也呈现均匀下降,但各基因型分布差异无统计学意义(P=0.782)。结论我国汉族人群DC-SIGN基因多态性变异很低,其对于今后HIV-1人群不易感性的研究意义不大;DC-SIGNR基因多态性对于HIV-1不易感性的意义尚需要大样本的验证或细胞生物学实验数据的支持。

Objective To study the genotypes and gene allele distribution of DC-SIGN and DC-SIGNR among HIV-1 carriers ,seronegative exposers （ESN） and healthy control,explore the correlation of low susceptibility to HIV-1 with gene polymorphism of DC-SIGN and DC-SIGNR. Methods The repeated sequences in neck region of DC-SIGN and DC-SIGNR were amplified and different alleles were identified using agarose gel electrophoresis. Results Genotype 7/7 was the primary genotypo of DC-SIGN. Only 9 non 7/7 genotype was tested in three groups. There was no significant difference between 7/7 and non-7/7 genotypes （P=0.648）. DC-SIGNR genetic polymorphism was high,and the frequency of 7/5,5/5 and 6/7 genotypes among ESN,healthy control and HIV-1 carriers gradually decreased. The allele frequency of 5 and 6 also showed a decreasing trend. But there no significant differences were observed （P=0.782）among the three groups. Conclusion Han Chinese has very low mutation frequency in DC-SIGN. It is not necessary to analyze DC-SIGN polymorphism in future study. Large number samples or data of cell biology were needed for verify the correlation of suscptibility of the target group to HIV-1 with DC-SIGNR polymorphism.