甘珀酸钠对小鼠急性肝损伤保护作用的研究

Protective Effect of Carbenoxolone Sodium on Acute Hepatic Injury

目的探讨甘珀酸钠(carbenoxolone sodium,CS)对四氯化碳(CCl4)所致小鼠急性肝损伤的保护作用。方法昆明种小鼠60只随机分为6组,各组分别每天给予不同剂量(160、80、40mg.kg-1)的CS和联苯双酯滴丸(200mg.kg-1)灌胃,对照组和模型组给予生理盐水。7d后,除空白对照组外各组小鼠皮下注射0.1%CCl4制备急性肝损伤模型,16h后取血检测血清ALT、AST活性;测定肝组织匀浆中MDA含量和SOD、GSH的活性及观察肝脏病理学变化。结果CS(160、80mg.kg-1)能降低CCl4所致急性肝损伤小鼠血清ALT、AST活性(P<0.01),降低肝脏MDA的含量(P<0.01),增强GSH和SOD活力(P<0.01,P<0.05),并能明显改善肝组织的病理学损伤。结论CS对CCl所致小鼠急性肝损伤具有保护作用。

Objective To explore, the protective effects of carbenoxolone sodium（CS） on acute hepatic injury induced by carbon tetrachloride （CCl4） in mice. Methods Sixty Kunming mice were randomly divided into 6 groups. The CS groups were treated with CS at a dose of 40, 80 and 160 mg·kg^-1·d^-1 for 7 days. At the same time, the normal control group and the model control group took equivalent amount of 0.9% sodium chloride solution by the same route. And mice in the positive control group were treated with 200 mg·kg^-1·d^-1 biphenyl dimethyl diearboxylate. After the last administration, all groups except the normal control were injected with 0.1% CCl4 solution to induce acute hepatic injury. Thereafter, the contents of ALT,AST in serum; MDA ,the activity of SOD and GSH in the liver were measured and the hepatic histological changes were observed by optical microscope. Results the contents of ALT, AST in serum was significantly decreased（ P 〈 0.01 ）, the content of MDA in the liver was also significantly lowered（ P 〈 0.01）, and the activity of GSH and SOD in liver was significantly improved after preventive treatment with CS（ P 〈 0.05）. CS ameliorated the hepatic pathological changes. Conclusion CS have protective effects on acute hepatic injury induced by CCl4 in mice.