RUNX3、DNMT1 mRNA在原发性肝细胞癌中的表达及其意义

Expression and Clinical Significance of Runt-related Ttranscription Factor 3 Gene and DNA Methyltransferase 1 in Primary Hepatocellular Carcinoma

目的研究人RUNT相关转录因子3(Runt-related transcription factor3,RUNX3)和DNA甲基转移酶1(DNA methyltransferase1,DNMT1)在原发性肝细胞癌(primary hepatocellular carcinoma,HCC)中的表达及其与临床病理学特征的关系,并探讨其临床意义。方法应用逆转录聚合酶链反应(Reverse transcription-Poly-merasechain reaction,RT-PCR)检测43例HCC患者癌组织及其相应癌旁组织中RUNX3mRNA、DNMT1mR-NA的表达。结果肝癌组织中RUNX3表达明显降低,DNMT1表达增高;RUNX3表达下调与组织分化程度有关(P<0.05),而与性别、年龄、HBsAg、AFP、静脉癌栓及TNM分期均无明显关系(P>0.05);DNMT1表达升高与静脉癌栓、组织分化程度及TNM分期有关(P<0.05),而与性别、年龄、HBsAg、AFP均无明显关系(P>0.05)。RUNX3与DNMT1表达成负相关(r=-0.2059,P<0.05)。结论RUNX3基因在肝癌组织中表达下调,DNMT1表达增高,可能与肝癌的发生发展有关;高表达的DNMT1在一定程度上参与调控RUNX3基因的表达下调。

Objective To investigate the expression and clinical significance of Runt-related transcription factor 3 gene（Runx3）and DNA methyhransferase 1（Dnmt1） in primary hepatocellular carcinoma （HCC）.Methods Reverse transcription-PCR（RT-PCR）was used to detect Runx3 mRNA , Dnmtl mRNA expression level of samples of primary hepatocellular carcinoma and corresponding para-cancer tissue, which were obtained from surgically resection specimens of 43 patients. Results RT-PCR showed that the expression of Runx3 in hepatocellular carcinoma was significantly lower than that in corresponding para-cancer tissue （0.2148 ± 0.0987 vs 0.6013 ±0. 1965, P 〈 0.05） and the expression of Dnmtl in hepatocellular carcinoma was higher than that in corresponding para-cancer tissue（0. 7425±0.4331 vs 0.4911 ±0. 1897, P 〈 0.05）. The Down-regulated expression of Runx3 was correlated with pathologic differentiation degree（ P 〈 0.05）. The up-regulated expression of Dnmtlwas correlated with vein tumor thrombus , TNM stage and pathologic differentiation degree（ P 〈 0.05）. The relationship between Runx3 and Dnmtl expression was significantly negative （ r = -0. 2059, P 〈 0.05 ）. Conclusion The down-regulated expression of Runx3 gene in human hepatocellular carcinoma suggest that Runx3 gene may play an important role in the development and progression of hepatocellular carcinoma. The up-regulated expression of Dnmtl participates to a certain extent degree in the inactivation of Runx3 expression.