六味地黄丸中四种活性成分的HPLC法测定

Determination of Four Effective Constituents in Liuwei Dihuang Pills by HPLC

建立了HPLC法同时测定六味地黄丸中的丹皮酚、马钱苷、莫诺苷和芍药苷。采用C18色谱柱,以乙腈-0.1%磷酸溶液梯度洗脱,检测波长238nm。丹皮酚、马钱苷、莫诺苷和芍药苷分别在0.5～20、0.25～10、0.5～20和0.15～6μg/ml浓度范围内线性关系良好,回收率分别为98.10%～105.44%。

An HPLC method was established for the simultaneous determination of paeonol, loganin, morroniside and paeoniflorin in Liuwei Dihuang pills. A C18 column was used with the mobile phase of acetonitrile-0.1% phosphoric acid for gradient elution at the detection wavelength of 238 nm. The calibration curves of the four constituents were linear in the concentration ranges of 0.5 - 20, 0.25 - 10, 0.5 - 20 and 0.15 - 6 μg/ml, respectively. The recoveries were 98.10% - 105.44%.