马尾松苯丙氨酸解氨酶基因的克隆与序列分析

Molecular cloning and sequence analysis of phenylalanine ammonia-lyase cDNA from Pinus massoniana

苯丙氨酸解氨酶(Phenylalanine ammonia lyase,PAL)是苯丙烷类代谢的关键酶,催化苯丙氨酸转化为肉桂酸,促进黄酮、木质素等次生代谢产物的合成。根据其他植物PAL基因的DNA保守序列设计的简并引物,经过PCR扩增,得到1条864 bp的特异性片段,编码288个氨基酸。通过分析其序列和编码的氨基酸序列,发现其与其他物种的PAL基因高度同源,证实为马尾松PAL基因的核心序列。所得序列已经登陆在NCBI的Genbank中,序列号为GQ142010。

Phenylalanine ammonia-lyase（PAL） plays a crucial role in phenylpropanoid metabolism and provides precursors of various phenylpropanoid compounds.According to the conservative domain of some other plants DNA sequences of PAL gene,a pair of degenerate primers was designed.A special DNA fragment was obtained by PCR amplifying,which length is 864 bp and it encoded a 288 amino acid protein.We analysed the nucleotide and amino of similarity to the corresponding gene of other organism,and the results show that it is a partial PAL gene of Pinus massoniana.The sequence was enrolled in the GenBank of NCBI.The accession number is GQ142010.