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桑树内生真菌Macrophomina phaseolina MOD-1产油脂培养基及摇瓶发酵条件的优化试验 被引量:5

Optimization of Culture Medium and Fermentation Conditions for Lipid Production from Mulberry Endophytic Macrophomina phaseolina MOD-1
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摘要 从健康桑树根系中分离筛选得到一株内生产油脂真菌——菜豆壳球孢菌MOD-1(Macrophomina phaseolina,MOD-1)。结合均匀设计和单因子筛选法得到该菌株产油脂发酵培养基最优配方为:可溶性淀粉105 g/L,蛋白胨1.1 g/L,磷酸二氢钾1.5 g/L,硫酸铵0.3 g/L,硫酸镁0.32 g/L,氯化锰4.9 nmol/L。在此基础上优化该菌株产油脂的摇瓶发酵条件为:发酵液初始pH7.0,发酵温度26℃,摇瓶转速190 r/min,装液量100 mL。在优化后的培养基组分及摇瓶发酵条件下培养6 d,菌体生长量(干质量)高达41.852 g/L,油脂产量达到25.511 g/L。利用气相色谱-质谱法分别测定菌株在优化后的培养基或发酵条件下产生油脂的脂肪酸组成,前一种条件下检测出9种脂肪酸成分,其中单不饱和脂肪酸含量为67.92%;后一种条件下检测出7种脂肪酸成分,其中单不饱和脂肪酸含量为74.32%。结果表明,在优化培养基及摇瓶发酵条件下,MOD-1菌株的油脂产量增高,组成简单,易于纯化,且单不饱和脂肪酸含量较高。 Macrophomina phaseolina MOD-1 is an endophytic oleaginous fungus isolated from the healthy mulberry root.To optimize medium composition and fermentation conditions for lipid production from MOD-1,the experiments with single factor and uniform design were conducted.The best medium composition was found with soluble starch 105 g/L,peptone 1.1 g/L,KH2PO4 1.5 g/L,(NH4)2SO4 0.3 g/L,MgSO4 0.32 g/L,and MnCl2 4.9 nmol/L.Based on this culture medium,the optimal fermentation conditions were as follows: initial pH 7.0,temperature 26 ℃,rotation speed 190 r/min and medium volume 100 mL.After being cultured for 6 h under the optimized condition,the culture's dry cell weight was found as high as 41.852 g/L and lipid yield reached 25.511 g/L.Determination of fatty acid composition in the cultures obtained under optimized culture medium or fermentation condition by gas chromatography and mass spectrometry(GC-MS) revealed that there were 9 kinds of fatty acids in the lipids from the former condition,among which the content of monounsaturated fatty acids was 67.92%,and there were 7 kinds of fatty acids in the lipids from the latter condition,among which the content of monounsaturated fatty acids was 74.32%.The results indicate that,under optimized condition,lipid yield of MOD-1 is increased.Its lipid composition is simple and easy to be purified.Besides,it has a high content of monounsaturated fatty acids.
出处 《蚕业科学》 CAS CSCD 北大核心 2010年第1期12-19,共8页 ACTA SERICOLOGICA SINICA
基金 山东省自然科学基金项目(No.Y2007D25 Q2008D08) 国家大学生创新性实验计划项目(No.081043407)
关键词 菜豆壳球孢菌MOD-1菌株 微生物油脂 培养基组分 发酵条件 均匀设计 脂肪酸组成 Macrophomina phaseolina MOD-1 Microbial lipid Medium composition Fermentation condition Uniform design Fatty acid composition
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