香菇柄多糖的微波辅助提取及其活性研究

Study on Microwave-assisted Extracion and Biological Activity of Polysaccearide from Lentinus Edodes Stem

对微波辅助提取香菇柄多糖的工艺进行研究,采用正交试验得出优化的工艺条件为:微波功率700W,液料比20∶1(mL∶g),提取时间3min,提取2次。与常规回流提取工艺相比,提取时间缩短60倍,多糖提取率提高88.7%。红外光谱检测表明微波辅助提取法和常规回流提取法所得多糖(分别为MAE-P和CRE-P)的红外图谱图具有比较相似的特征;MAE-P对S180抑瘤率为35.63%,并且对脾淋巴细胞有极显著增殖作用,其活性与CRE-P相比无显著差异。结果表明:微波辅助提取法不仅促进香菇柄多糖的有效溶出,而且能够较好地保持其生物活性。

Microwave-assisted extraction （MAE） of polysaccharide from Lentinus edodes stem was studied with orthogonal test to obtain the best extraction conditions： microwave power 700 W, liquid to solid ratio of 20：1 （mL：g）, extraction time for 3 min, two times. By comparing with the conventional reflux extraction, extraction time of MAE was about less than 60 times, the polysaccharide yield was increased by 88.7 %. The polysaccharide （MAE-P and CRE-P, respectively） collected by two extraction methods had similar IR spectra. Inhibition rate of MAE-P against Slso solid tumor was 35.63 %and MAE-P had very significant effect on promoting spleen lymphocytes cell proliferation, there was no significant difference in biological activity between MAE-P and CR-P. The results showed that the microwave-assisted extraction has not only promoted the effective dissolution of polysaccharide, but also better maintained its biological activity.