GC检测虾中氯霉素残留的研究

The Study on Determination of Chloramphenicol Residues in Shrimp by Gas Chromatography

建立了虾中氯霉素残留的带电子捕获检测器的气相色谱方法。通过改进虾样品前处理的操作，结果表明：氯霉素浓度在0．5μg/L～500μg/L的范围内呈良好的线性关系（R^2=0．9996），最低检测限为0．1μg／kg，定量限为0．5μg／kg；向样品中分别添加10、100、200μg／kg3个浓度水平的氯霉素回收率分别为99．3％、109．9％和116．9％，变异系数为3．1％～9．2％。该方法灵敏，精密度高。

A method had been established for the determination of chloramphenicol residues in shrimp by gas chromatography with electron capture detection. The measure about pre-process shrimp had been improved. The results showed that the calibration curves of chloramphenicol were linear（R2=0.999 6） in the range of 0.5 μg/L- 500μg/L. The limit of detection and quantification respectively was 0.1 μg/kg and 0.5 μg/kg. The average recoveries were 99.3 %, 109.9 % and 116.9 % respectively when samples spiked with 10,100 and 200 μg/kg. The conficient of variation was 3.1%-9.2 %. The method is sensitive and accurate.