板蓝根提取物中核苷类成分的纯化工艺研究

Purification technology of total nucleotide separated from extract of Radix Isatis

目的研究板蓝根提取物中核苷类成分的纯化工艺。方法采用UV法测定总核苷的含量,通过比较4种大孔树脂的性质,选择HPD450型大孔树脂作为纯化树脂,并对纯化工艺各项影响因素进行了考察。结果确定HPD450大孔树脂纯化核苷类成分的最佳纯化工艺为:树脂柱的径高比为1∶7,以1.5 BV.h-1的流速上样,上样后先用1.5倍柱体积蒸馏水洗脱、再用5倍50%乙醇以1.5 VB.h-1洗脱。经最佳纯化工艺纯化,得到板蓝根提取物总核苷的纯度>50%。结论此纯化工艺可制备核苷类成分纯度较高的板蓝根提取物。

Objective To study the purification technology of total nucleotide separated from extract of Radi：c Isatis, Methods UV was employed to determine the total nucleotide content. Through comparing the properties of 4 macroporous asorption resins, HPD450 macroporous asorption resin was chosen to separate total nucleotide, and several factors affecting the purification technology were investigated. Results The purification technology of total nucleotide was as follows： the ratio of the diameter and height of the resin column was 1 ： 7, flow velocity of sample solution was 1.5 BV ·h-1 , eluting the resin at 1.5 BV water and 5 BV 50% ethanol by turns. The purity of total nucleotide in purified extract of Radicc Isatis was over 50%. Conclusion Higher purity total nucleotide of Radix Isatis extract can be prepared with the purification technology.