摘要
目的建立测定清热养阴颗粒(百合、知母)中芒果苷含量的RP-HPLC法。方法采用ZORBAX-C18柱(4.6mm×250mm,5μm),流动相:甲醇-乙腈-1%冰醋酸溶液(10:5:85),流速:0.8mL·min-1,柱温:30℃,检测波长:318nm。结果芒果苷在0.012-0.240mg·mL与峰面积有良好线性关系(r=0.9995),平均加样回收率为99.3%,RSD〈2.0%。结论本方法简便、快速、准确、重复性好,可用于该制剂的质量控制。
Objective To establish an RP HPLC method for the content determination of mangifefin in Qingreyangyin granules (Bulbus Lili, Rhizorna Anemarrhenae ). Methods ZORBAX-CI8 (4.6 mm × 250 ram, 5 μm) column was used with methanol-acetonitrile-1% glacial acetic solution (10 : 5 : 85) as the mobile phase, the flow rate was 0. 8 mL · min 1, and the column temperature was 30 ℃. The UV detector was 318 nm. Results The linear range was 0. 012- 0. 240 mg ·mL (r=0. 999 5). The average recovery of mangiferin was 99.3%, and RSD〈2.0%. Conclusion The It can be used in the determination of mangiferin in Qingreyangyin
出处
《中南药学》
CAS
2010年第2期131-133,共3页
Central South Pharmacy