奥曲肽体外对黑色素瘤细胞株A375分泌VEGF的影响

Research of the effect of octreotide on cultured A375 melanoma cell lines

目的:了解奥曲肽对体外培养黑色素瘤细胞株A375的影响。方法:体外培养扩增A375细胞,分别与浓度为5,1,0.2,0mg/L的奥曲肽共培养24,48,72h,利用MTT法测定各组奥曲酞对A375细胞增殖作用的影响,通过ELISA法测定奥曲酞对A375细胞分泌VEGF作用的影响。结果:共培养24h后,奥曲酞对A375细胞增殖有显著促进作用(F=14.180,P=0.00),48h及72h增殖作用不明显;奥曲酞浓度及作用时间对体外培养的A375细胞分泌的VEGF含量有显著性影响(浓度因素:F=24.441,P=0.00;时间因素:F=127.233,P=0.00),各浓度的奥曲酞与A375细胞共培养后24h均呈现明显的增加细胞分泌VEGF的作用,浓度越高增加的越少(单向方差分析F=19.489,P=0.00);随着作用时间的延长,细胞分泌VEGF减少,72h各浓度的奥曲酞均明显抑制A375细胞分泌VEGF,浓度越高抑制作用越明显(单向方差分析F=16.116,P=0.002)。结论:奥曲肽对黑色素瘤细胞A375分泌VEGF长期的趋势上是抑制作用,与时间和剂量呈依赖关系。

AIM： To understand effects of octreotide on vascular endothelial growth factor （VEGF） secretion in cultured A375 melanoma cells. METHODS： A375 cells in vitro amplificated were co- cultured with octreotide of concentration 5,1 and 0.2mg/L for 24,48 and 72 hours. Whether octreotide affected the proliferation of A375 melanoma cells was tested by MI-F methods. The octreotide effects on VEGF secretion in A375 cells were tested according to the ELISA method, and octreotide effects on cultured A375 melanoma cell line proliferation and VEGF secretion were statistically analyzed. RESULTS： Twenty-four hours after coculture, octreotide improved the A375 cell proliferation （ F= 14. 180, P = 0. 00）. The concentration and time of octreotide coculture with A375 cells in vitro affected the VEGF secretion significantly （ content factor： F = 24. 441, P = 0. 00; time factor： F = 127. 233, P = 0. 00）. Octreotide of the three concentrations improved VEGF secretion in A375 cells in 24 hours. The higher concentration, the less in crease of VEGF level（ One-way analysis of variance： F = 19. 489, P = 0.00）, and octreotide decreased VEGF secretion in A375 cells in 72-hour, the inhibiting power accorded with the concentration（ One-way analysis of variance： F= 16.116, P = 0.002）. CONCLUSION： Octreotide inhibition on VEGF secretion in A375 melanoma cells is a long-term trend and in a dose-dependent manner.