血小板衍生生长因子-D在肝细胞肝癌中的表达及其临床意义

Expression and Clinical Significance of Platelet-derived Growth Factor-D(PDGF-D) in Hepatocellular Carcinoma

目的研究血小板衍生生长因子(PDGF)D在肝细胞肝癌(HCC)中的表达及其与临床病理指标的关系。方法用RT-PCR方法检测PDGF-A、B、C、D及其受体PDGFRα和PDGFRβ基因在40例肝癌组织及对应的30例癌旁组织和10例正常肝组织标本中mRNA的表达。结果HCC组织中PDGF-A、B、C、D及其受体PDGFRα和PDGFRβ阳性表达分别为37.5%(15/40)、70%(28/40)、52.5%(21/40)、92.5%(37/40)、22.5%(9/40)、42.5%(17/40);与其相对应的癌旁组织的阳性表达率分别为23.3%(7/30)、16.7%(5/30)、16.7%(5/30)、26.7%(8/30)、13.3%(4/30)、16.7%(5/30);而正常肝组织中未见表达。在HCC组织中PDGF家族的表达均显著高于癌旁组织和正常组织,三组比较差异有统计学意义(P<0.01),且PDGF-D的阳性表达显著高于PDGFs其他亚型(P<0.05)。在HCC、癌旁、正常肝脏组织中PDGF-D平均光密度值为0.5900±0.0364、0.3954±0.0654、0,三者比较差异有统计学意义(P<0.01)。HCC组织中PDGF-D的阳性表达与肿瘤的分化程度、淋巴及转移有关(P<0.05);与年龄、性别、肿瘤大小、血清AFP的值及门静脉癌栓无关(P>0.05)。结论PDGF家族中以PDGF-D表达率最高且与肿瘤的分化程度和转移相关,提示PDGF家族,尤其是PDGF-D在HCC的发生、发展和转移中可能发挥着重要的作用,可作为肝癌辅助诊断指标和治疗靶点。

Objective To investigate the expression and clinical significance of Platelet-Derived Growth Factor-D（PDGF-D） in hepatocellular carcinoma （HCC）. Methods PDGF-A,B,C,D and PDGF receptor （PDGFR）α and β mRNA level was assessed by reverse transcription-polymerase chain reaction（RT-PCR）in 40 cases HCC tissues,30 cases of paracarcinoma tissues and 10 cases of normal liver tissues.Results PDGF-A,B,C,D and PDGFRα,β mRNA level was 37.5%,70.0%,52.5%,92.5%,22.5%,42.5% in HCC, 23.3%,16.7%,16.7%,26.7%,13.3%,16.7% in paracarcinoma tissues respectively.No expression of PDGF-D mRNA was found in the normal liver tissues.PDGF-D mRNA expression was significantly higher than that in paracarcinoma tissues and normal liver tissues,there were significantly differences among three groups（P〈0.01）.Moreover, the expression of PDGF-D mRNA was significantly higher than PDGF other isoforms in HCC tissues（P〈0.05）.Average photodensity of PDGF-D were 0.5900±0.0364,0.3954±0.0654 and 0 in HCC,paracarcinoma and normal liver tissues,there were significantly differences among three groups（P〈0.01）.PDGF-D mRNA expression were related to tumor differentiation degree,metastasis,but not to gender,age,tumor size and portal vein cancer embolus（P〉0.05）. Conclusion PDGF-D mRNA expression was significantly higher in HCC and were related to tumor differentiation degree and metastasis.PDGF-D may play an important role in the carcinogenesis,development and metastasis of HCC,as a clinical diagnosis index and a potential target for therapy in HCC.