不同强度光照对母鸡中脑和间脑c-fos基因表达的影响

Influence on the Expression of c-fos Gene by the Different Intensity Light Stimulation in Hen Mesencephalon and Diencephalons

为进一步理解光照对母鸡产蛋性能的影响机制,此研究用c-fos法探讨了母鸡对不同强度光照刺激的反应。将150日龄的母鸡右眼遮光7天后接受不同强度光照刺激1.5h,暗适应1.5h后灌流固定,取脑制作石蜡切片,采用免疫组化方法检测c-fos基因在中脑和间脑的表达。结果显示:间脑和中脑内有大量Fos样免疫反应阳性神经元,主要见于左侧,分布于视顶盖中央灰质层(SGC),圆核(ROT),外侧膝状腹侧核(Glv),半月核(SLu),峡核大细胞部(Imc),狭核小细胞部(Ipc),室旁核(PVN),弓状核(ARC),动眼神经核(OMv)。不同强度光照诱导母鸡c-fos基因的表达不同,在SGC,ROT,SLu,Imc,Ipc,Glv,20 lx,30 lx组的表达较对照组明显增加,40 lx组次之,10 lx组最低;PVN,ARC为20 lx组表达最强,30 lx,40 lx组逐渐减弱;而OMv的表达随着强度的增加而加强。

In order to further know the mechanism in the effect of light on egg-laying in hen,the response of hen brain on different light intensity was investigated using c-fos method.The right eyes of 150 days old hens were weared a blinder for 7 days,and received the different intensity light stimulation for 1.5 hours and dark adaptation 1.5 hours.After perfusion with fixative,their brains were removed from the skulls and were made into continuous paraffin sections.Using immunohistochemical method,expression of c-fos gene in mesencephalon and diencephalons was examined.The result showed that a number of Fos-like immunoreactive positive neurons were observed in hen mesencephalon and diencephalons after a light stimulation,being mainly present in left stratum griseum centrale（SGC） of tectum,nucleus rotundus（ROT）, nucleus geniculatus lateralis pars ventralis（GLV）,nucleus semilunaris（SLu）,isthmi pars principals（Imc,Ipc）, paraventricular nucleus（PVN）,arcuate nucleus（ARC）,and nucleus nervi oculomotorii（OMv）.The expression of c-fos gene was varied with the different intensity light stimulation in hen,the immunoreactive intensity was the highest in 20 lx and 30 lx group,secondary in 40 lx,the lowest in 10 lx group within SGC,ROT,SLu,Glv, Imc and Ipc.That was highest in 20 lx group,gradually weakened in 30 lx and 40 lx group within PVN and ARC.The immunoreactive intensity was increased with the intensity of light within OMc.