自由人槭‘秋焰’(Acer×freemanii‘Autumn Blaze’)组培的褐变控制研究

Studies on Browning Control in Tissue Culture of Acer×freemanii‘Autumn Blaze’

针对自由人槭‘秋焰’(Acer×freemanii‘Autumn Blaze’)初代培养外植体褐化严重问题,采取不同取材时间、添加抗褐变剂、暗处理等措施,研究了三种措施对自由人槭‘秋焰’组织培养中褐化的影响效应。结果表明:随着取材月份变化,外植体褐化率提高;AC和PVP对褐变的处理,与Vc和Na2S2O3的处理相比达到差异极显著,又以2.0g/L的PVP抑制褐化效果最佳;随着黑暗处理时间的加长,褐化率呈现高-低-高的变化趋势。春季4、5月份取材,接种于添加PVP2.0g/L的MS上时,直接进行光照培养,可有效控制褐变。

The measures including different collecting time,adding antibrowning reagents and culturing in darkness were carried out in terms of severe browning phenomenon in the process of Acer×freemanii‘Autumn Blaze’tissue culture.The effects of three browning control measures were studied in the paper.The results were as follows：The browning rate increased with months changed;The antibrowning effects with AC and PVP showed extremely significant difference with contrast to that of Vc and Na2S2O3.The browning control effects of PVP with concentration of 2.0 g/L was optimal;The browning rate presented higt-low-high tendency with the darkness time increased.Measures of collecting explants in April and May,inoculating on MS adding 2.0 g/L PVP,culturing under the light directly could control browning effectively.