Sr胁迫对油菜幼苗抗氧化指标影响的研究

EFFECTS OF STRONTIUM STRESS ON ANTIOXIDANT CHARACTERISTICS OF OILSEED RAPE SEEDLING

在石英砂和Hoagland营养液培养条件下,用浓度为0、10、20和40mmol/L的88Sr2+(88SrC l2)处理油菜幼苗(Brassica napus L.)(三叶期到六叶期),动态研究Sr胁迫对油菜幼苗抗氧化指标的影响,以及Sr2+积累量和富集能力的变化。结果表明,油菜幼苗对Sr2+表现出高富集能力,富集能力依次为:叶>茎>根,Sr2+浓度越高,富集能力越低。与对照相比,Sr2+胁迫下的油菜超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量随Sr2+浓度的增加及胁迫时间的延长呈先上升后下降趋势;过氧化物酶(POD)和过氧化氢酶(CAT)活性则随富集Sr2+能力的提高而降低。因此得出结论,油菜幼苗对Sr胁迫具有较高的耐受能力和富集能力;叶片是最主要的富集器官;富集能力与POD,CAT活性显著负相关。

Seedlings of oilseed rape（Brassica napus L.） were treated with different concentration of Sr^（2＋）（0,10,20,40mmol/L SrCl_2） for 0,7,14,21days under quartz sand and Hoagland solution culture,and the antioxidant characteristics and Sr^（2＋）accumulation of seedling were investigated.Rape seedlings showed high accumulation capacity for strontium,especial the leaves.But the accumulation capacity declined with the increasing of Sr^（2＋） concentration.The activity of superoxide dismutase（SOD） and the content of malondialdehyde（MDA） increased with the increasing of Sr^（2＋） concentration and then declined with the extention of the stress time.The activity of catalase（CAT） and peroxidase（POD） declined with the increasing of the Sr^（2＋）accumulation capacity.This study showed that oilseed rape had a very high strontium-tolerant and strontium-uptake capacity,and leaf was the organ that accumulates most of strontium.The accumulation capacity and activity of CAT,POD exhibited a significantly-negative correlation.