高效液相色谱手性固定相法拆分阿折地平对映体

Enantiomeric separation of azelnidipine by high performance liquid chromatography with chiral stationary phase

建立了阿折地平对映体的高效液相色谱拆分方法。采用ChiralpakAD-H(250mm×4.6mm,5.0μm,Dai-cel公司)手性色谱柱在正相条件下直接拆分阿折地平对映体,考察了固定相种类、流动相组成及柱温等对阿折地平对映体分离的影响。确定了最佳的拆分条件:流动相为正己烷-异丙醇(90:10,v/v),流速为0.8mL/min,检测波长为254nm;柱温为20℃;在此条件下阿折地平对映体的分离度为3.3。该法简单快速,重现性好。

A high performance liquid chromatographic（HPLC） method was established for the enantiomeric separation of azelnidipine.Baseline chiral separation of azelnidipine was achieved under normal-phase chromatographic mode using the Chiralpak AD-H chiral column（250 mm×4.6 mm,5.0 μm,Daicel）.The influences of the nature of chiral stationary phase,form of the mobile phase,and column temperature on the enantiomeric separation were studied.The optimized chromatographic conditions were hexane-isopropyl alcohol（90∶10,v/v） as the mobile phase with a flow rate of 0.8 mL/min and detection at the wavelength of 254 nm.The column temperature was set at 20 ℃.The resolution of 3.3 for azelnidipine was achieved under the above chromatographic conditions.The method is simple,rapid and with good reproducibility.