摘要
目的:探讨局部应用辛伐他汀促进拔牙术后牙槽骨修复机制。方法:选用健康雄性Wistar大鼠56只,随机分为实验组28只和对照组28只,拔除右下颌中切牙后,实验组即刻植入载辛伐他汀PLGA支架材料,对照组植入单纯PLGA作为对照,于术后5d、1周、2周和4周分别处死大鼠,设计针对VEGF mRNA的寡核苷酸探针,采用原位杂交技术检测拔牙窝VEGF mRNA的表达。结果:术后1周和2周实验组VEGF阳性细胞数和染色强度均高于对照组,且差异有统计学意义,而术后4周,实验组和对照组阳性细胞数无统计学差异,且阳性表达细胞少于术后2周。结论:局部应用辛伐他汀通过增加VEGF mRNA的表达促进拔牙窝骨损伤的愈合。
Objective: To investigate the mechanism of topically used Simvastatin on promoting alveolar bone repair following tooth extraction. Methods: Fifty eight male Wistar rats were divided into experimental groups and control groups (28 rats/group). PLGA scaffold was immediately implanted with or without Simvastatin in extraction sockets of the mandibular incisors. Hybridization technique was conducted after 5days, lweeks, 2weeks and 4weeks. Results: The number of VEGF positive vascular endothelial cells in experimental group was significantly higher than that in control group after 1 and 2 weeks of implantation(P〈0.05). After 4 weeks of implantation, the number of VEGF--positive cells in experimental group was not significantly higher than that of control group (P〈 0.05). Conclusion: Simvastain promotes alveolar bone repair following tooth extractiontby up--regulation of VEGF by mRNA expression.
出处
《口腔医学研究》
CAS
CSCD
北大核心
2010年第1期4-6,共3页
Journal of Oral Science Research
基金
国家自然科学基金项目(编号:30872912)