异种脱细胞真皮基质引导即刻种植种植体周围骨缺损再生能力的实验研究

Experimental Study of the Acellular Dermal Matrix as a Membrane Used for Guided Bone Regeneration around Dental Implants in Immediate Implant

目的:运用异种脱细胞真皮基质进行引导骨组织再生术(guided bone regeneration,GBR)评价修复种植体周围骨缺损能力,为临床应用提供指导。方法:在4只成年Beagle犬下颌第2、3、4前磨牙新鲜拔牙创即刻植入种植体,并在颊侧形成3mm×3mm×5mm骨缺损区,按自身同期对照研究设计,右侧为实验侧,骨缺损区上覆盖海奥膜;左侧为空白对照侧,骨缺损区不覆盖海奥膜。术后1、4个月分别处死一组动物,摘取下颌骨,采用大体观察、X线摄片、组织学观察测定等方法检测缺损区骨组织再生的情况。结果:实验侧种植体周围骨缺损区较空白对照侧新骨生成量多,加速了骨组织的再生过程。结论:异种脱细胞真皮基质具有良好的生物相容性和可降解性,可用作骨组织引导再生膜,促进骨缺损的再生修复。

Objective： To investigate the effects of guided bone regeneration（GBR） technique with acellular dermal matrix membrane in repairing bone defects around dental implants in immediate implant. Methods： In 4 beagle dogs, pure titanium implants were immediately implanted after extraction of the second, third and fourth premolars of both sides in mandibles, and standard bone defects of 3mm×3mm×5mm were created at buecal side of the implants. The defects on the right side were covered with ADM membrane, while the left side was uncovered as control. The dogs were sacrificed after 1,4 months. The specimens were removed and studied by gross observation, X --ray radiograph and histological examination. Results： The quantity and quality of new bone formation on experi mental side where bone defects were covered with ADM membrane were much better than that on the control side. Conclusion： ADM membrane has excellent biocompatihility and biodegradablility and can be used in guided bone re generation to promote the bone repair progress.