葡萄雄性不育新种质2004-6-12(魏可实生)花蕾发育的生化分析

Biochemical Analysis of Male Sterile Newly-bred 2004-6-12(Wink Seedling) during Flower Development

以葡萄雄性不育种质2004-6-12(魏可实生)及魏可为试材,测定了不同发育时期花蕾的可溶性糖、可溶性蛋白质、脯氨酸含量以及过氧化物酶(POD)、超氧化物歧化酶(SOD)活性。结果表明,随着花蕾不同时期的发育,2004-6-12(魏可实生)在各个时期可溶性蛋白质、脯氨酸含量均低于魏可,可溶性糖含量单核晚期以前低于魏可,双核期后高于魏可,表明这些生化指标与小孢子发育有密切的联系;POD活性显著小于魏可,且呈降低趋势,SOD活性则与之相反。

The male sterile newly -bred 2004 -6 -12 （Wink seedling） and Wink rape were taken as experimental materials and the soluble sugar, soluble protein and proline content in anther, as well as the POD and SOD activity were tested. The results showed that the content of soluble protein and proline in 2004 -6 - 12 （Wink seedling） was lower than that in Wink during flower develop- ment, the content of soluble sugar was lower than that in Wink before the late mononuclear stage, but it was higher than that in Wink after binuclear stage, which indicated that these indexes were closely related to the microspore development. Meantime, the POD ac- tivity of 2004 -6 - 12 （kWink seedling） was decreased and markedly lower than that of Wink, while the SOD activity showed the reverse way.