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新型有序纳米介孔生物活性玻璃体外复合培养成骨细胞生物相容性研究 被引量：3

The Study of the Mesoporous Bioactive Glass Biological Behavior in Stimulated Body Fluid
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摘要 目的评价新型有序纳米介孔生物活性玻璃(80SMBG)对成骨细胞的生物相容性。方法以Novabone为对照,用大鼠成骨细胞与80SMBG浸提培养液、Novabone浸提培养液和正常的空白培养液培养,分别于3、4、5d用四唑盐(MTT)比色法计数计算细胞相对增殖度,用6级毒性分类法评级,同时用偶氮偶联法(PNPP)染色,分析碱性磷酸酶活性的变化。结果80SMBG毒性评级为0～1级,对成骨细胞分化无明显的抑制作用。结论80SMBG的体外生物活性较高,对体液微环境的影响轻微,细胞相容性好,作为骨组织修复替代材料或骨组织工程支架材料具有较高的研究和应用价值。 Objective Evaluate the cytocompatibility ofmesoporous bioactive glass（80S MBG） in vitro. Method The eytotoxicity of 80S MBG was evaluated by the cultivation of mouse osteoblasts. We investigate cell proliferation and differentiation in contact with the ionic products from the dissolution of 80S MBG,Novabone and the normal empity culture medium. The samples were observed with MTT and ALP active at 3,4,5 days respectively. Result The toxicity gradation of 80S MBG was 0 - 1. Conclusion 80S MBG has better cytocompatibility. It may be used as bone-repaire and bonesubstitute materials in the future.
作者 陈建伟 张权 严晓霞 黄煌渊
机构地区 复旦大学附属华山医院骨科 上海交通大学医学院附属仁济医院骨科 复旦大学化学系无机材料合成实验室多孔材料课题组
出处 《生物骨科材料与临床研究》 CAS 2010年第1期16-19,23,共5页 Orthopaedic Biomechanics Materials and Clinical Study
基金 国家自然科学基金资助项目(30571877) 上海市自然科学基金(06ZR14127)
关键词 生物活性玻璃 生物活性 细胞相容性 Bioactive glass Bioactivity Cytocompatibility
分类号 R318.08 [医药卫生—生物医学工程]
  • 相关文献

参考文献9

  • 1Yan X, Deng HX., Huang XH, et al. Mesoporous bioactive glasses. I. Synthesis and structural characterization [J]. Journal of Non- Crystalline Solids,2005,3:1-9.
  • 2Yan X, Yu C, Zhou X, et al. Highly ordered mesoporous bioactive glasses with superior in vitro bone-forming bioactivities [J]. Angew Chem Int Ed Engl,2004,43(44):5980-5984.
  • 3陈建伟,张权,严晓霞,黄煌渊.新型有序纳米介孔生物活性玻璃在模拟体液中的表面生物活性研究[J].生物骨科材料与临床研究,2009,6(4):1-4. 被引量：6
  • 4郝和平.生物材料和生物制品的生物学评价标准[M].北京:中国标准出版社,2000,100.110.
  • 5章晔,张权,黄煌渊.新型有序纳米介孔生物玻璃材料对体外培养成骨细胞的影响[J].老年医学与保健,2008,14(2):95-99. 被引量：4
  • 6Yourtee MD,Tong PY,Rose LA ,et al.Effect of spiroorthoeabonate volume modifier comonomers on the in vitro toxicology of trial non-shrinking dental epoxy copolymers[J].Res Commun Mol Pathol Pharmacol. 1994.86(3 ):347.
  • 7张权,谢寒,余承忠,易静.新型有序纳米介孔生物活性玻璃对成骨细胞IGF-Ⅱ基因调控的影响[J].复旦学报（医学版）,2009,36(3):313-316. 被引量：5
  • 8Lossdorfer S, Schwartz Z, Lohmann CH, et al. Osteoblast response to bioactive glasses in vitro correlates with inorganic phosphate content[J]. Biomaterials, 2004,25(13):2547-2555.
  • 9Silver IA, Deas J, Ereciriska M. Interactions of bioaetive glasses with osteoblasts in vitro: effects of 45S5 Bioglass, and 58S and 77S bioactive glasses on metabolism, intracellular ion concentrations and cell viability[J]. Biomaterials, 2001,22(2): 175-85.

二级参考文献24

  • 1涂杰,王迎军,陈晓峰,欧阳钧,邱小忠.生物玻璃矿化性能及其离子溶出对成骨细胞功能的影响[J].无机材料学报,2007,22(1):123-127. 被引量：4
  • 2Yan X., Deng H.X., Huang X.H., et al. Mesoporous bioactive glasses. I. Synthesis and structural characterization [J]. Journal of Non-Crystalline Solids. 2005 ,Sep;3:1-9.
  • 3Yan X, Yu C, Zhou X, et al. Highly ordered mesoporous bioactive glasses with superior in vitro bone-forming bioactivities [J]. Angew Chem Int Ed Engl. 2004 Nov 12;43(44):5980-4.
  • 4Kokubo T, Kushitani H, Sakka S, et al. Solutions able to reproduce in vivo surface-structure changes in bioactive glass-ceramic A-W [J]. J Biomed Mater Res. 1990 Jun;24(6):721-34.
  • 5Chuang KS, Chen C Y, Yuan L J, et al. Shape - based grey - level image interpolation [J]. Phys Med Biol, 1999,44 (6):1565-77.
  • 6Grevera GJ, Udupa J K. Shaped - based interpolation of multidimen- sional grey - level images IEEE Trans [J]. Med Imaging, 1996,15 (6) : 881-92.
  • 7Higgins W E, Orlick C J, Ledell B E. Nonlinear filtering approach to 3- D gray - scale image interpolation [ J ]. IEEE Trans Med Imaging, 1996,15 (4) : 580-7.
  • 8M. M. Pereira, L. L. Hench, Mechanism of Hydroxyapatite Formation on Porous Gel-Silica Substrates [J]. J Sol-Gel Sci. Technol. 1996,7, 59.
  • 9M. M. Pereira, A. E. Clark, L. L. Hench, Effect of Texture on the Rate of Hydroxyapatite Formation on Gel-Silica Surface [J]. J. Am. Ceram. Soc. 1995, 78, 2463.
  • 10Yan X,Yu C,Zhou X,et al.Highly Ordered mesoporous Bioactive Glasses with Superior in vitro Bone-forming Bioactivitics.Angew Chem Int Ed Engl.2004,43:5980-5984.

共引文献9

同被引文献14

  • 1杨玉生,孙俊英.生物活性玻璃:促进骨修复的骨移植替代材料[J].中国临床康复,2005,9(42):108-112. 被引量：7
  • 2杨玉生,孙俊英,朱国兴.生物活性玻璃对鼠成骨细胞体外增殖的形态学研究[J].生物医学工程研究,2007,26(2):146-150. 被引量：11
  • 3郝和平.生物材料和生物制品的生物学评价标准[M].北京:中国标准出版社,2000,100.110.
  • 4Yan X,Deng HX,Huang XH,et al.Mesoporous bioactive glasses.I.Synthesis and structural characterization[J].Journal of NonCrystalline Solids,2005,3:1-9.
  • 5Yan X,Yu C,Zhou X,et al.Highly ordered mesoporous bioactive glasses with superior in vitro bone-forming bioactivities[J].Angew Chem Int Ed Engl,2004,43(44):5980-5984.
  • 6Valerio P,Pereira MM,Goes AM,et al.The effect of ionic products from bioactive glass dissolution on osteoblast proliferation and collagen production.Biomaterials.2004 Jul;25(15):2941-8.
  • 7Lossdorfer S,Schwartz Z,Lohmann CH,et al.Osteoblast response to bioactive glasses in vitro correlates with inorganic phosphate content[J].Biomaterials,2004,25(13):2547-2555.
  • 8Silver IA,Deas J,Ereci(n)ska M.Interactions of bioactive glasses with osteoblasts in vitro:effects of 45S5 Bioglass,and 58S and 77S bioactive glasses on metabolism,intracellular ion concentrations and cell viability[J].Biomaterials,2001,22(2):175-185.
  • 9Schmidt A, Ladage D, Schinkothe T, et al. Basic fibroblast growth factor controls migration in human mesenchymal stem cells[J]. Stem Cells,2006,24(7) : 1750-1758.
  • 10Frost HM. A determination of bone architecture. The minimun effective strain[J]. Clin Orthop Relat Res, 1983, (175):286- 292.

引证文献3

二级引证文献5

生物骨科材料与临床研究
2010年 第1期

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