前列腺癌细胞PC-3骨转移动物模型的建立

Establishment of bone metastasis model of prostate cancer celline PC-3

目的建立前列腺癌细胞PC-3骨转移动物模型,观察肿瘤在骨组织的局部生长及对骨组织的破坏情况,同时观察肿瘤远处器官转移情况。方法16只8周龄BALB/C裸鼠,分为实验组、对照组各8只。BALB/C裸鼠麻醉后,实验组裸鼠将10μl约2×105个PC-3细胞悬液从左侧胫骨近端关节面注入胫骨骨髓腔内,建立骨转移模型。对照组则注入10μl生理盐水。观察裸鼠的活动情况。6周后麻醉裸鼠行X线检查,观察骨破坏情况。然后处死裸鼠,取左侧下肢、肺和肝脏,经固定、石蜡包埋、切片及HE染色后在显微镜下观察组织学情况。结果实验组8只裸鼠骨转移模型建立均成功,其中7只X线检查表现出明显溶骨性破坏。解剖后发现其中6只在骨外也有明显的肿瘤组织生长。显微镜下观察8只裸鼠左侧胫骨内均有肿瘤生长,其中3只发生肺转移。对照组则均未发现明显骨质破坏。结论成功建立了前列腺癌细胞PC-3骨转移模型,模拟了前列腺癌对骨组织的侵袭和破坏及远处转移,为进一步研究前列腺癌对骨破坏的机制及治疗提供了基础。

Objective To establish bone metastasis model of prostate cancer cell line PC-3 for observing local growth and distant metastasis of the tumor and destruction of bone by the tumor. Methods Total 16 male nude mice of 8 weeks old were devided into two groups： experimental group and control group, 8 each group. For 8 mice in the experimental group, 10ul （2× 10^5 ）of sus pension of PC-3 cells were injected into left tibia of the mice. And for the control group, the same volume of normal saline were injected. Observe the activities of the mice. 6 weeks after the injection, mice were anesthetized and a plain film was taken to evaluate bone destruction of the tibia. The lungs, livers, and left hind legs were harvested after sacrifice. The specimens were fixed in 10% formalin, embedded in paraffin, stained by H＆E and observed microscopically. Results In the experimental group, tumor cells were found in all the tibia of the mice. Tumor growth out of the tibia were found in 6 mice. Lung metastasis were found in 3 mice. Bone destruction were found in 7 mice on X ray film. While in the control group, no tumor and no bone destruction were found. Conclusions Bone metastasis model of prostate cancer cell line PC-3 were successfully established. Tumor invasion, distant metastasis and bone destruction were found in the model. It is a useful tool for in vestigating mechanism of bone destruction and treatment of bone metastasis.