SRB法检测As_2O_3对KB细胞和Tca8113细胞毒性的研究

Cytotoxicity of Arsenic Trioxide on KB Cells and Tca8113 Cells by SRB Assay

目的:采用SRB(Sulforhodamine B)法研究三氧化二砷(As2O3)对人口腔鳞癌KB细胞和Tca8113的细胞毒性。方法:采用SRB法检测As2O3作用于KB细胞和Tca8113细胞前后的A值变化。结果:SRB法检测结果显示在KB组,1、1×10-1、1×10-2、1×10-3、1×10-4μg/mL质量浓度的As2O3所对应的A值分别为0.11、0.21、0.26、0.27、0.27;在Tca8113组,1、1×10-1、1×10-2、1×10-3、1×10-4μg/mL质量浓度的As2O3对应的A值分别为0.48、0.81、0.93、0.97、0.97。结论:As2O3对人口腔鳞癌KB细胞和Tca8113细胞的增殖有明显抑制作用,癌细胞抑制率呈现出剂量依赖性效应关系。

Objective：To detect the cytotoxicity of arsenic trioxide（As2O3） on the KB and Tca8113 cells line.Methods：Observing the changes of restrain of the KB and Tca8113 cells growth through SRB assay.Results：The SRB assay showed that in the KB group,the corresponding A values of 1,1×10-1,1×10-2,1×10-3,1×10-4 μg/mL concentrations of As2O3 were 0.11,0.21,0.26,0.27,0.27 respectively;in Tca8113 Group,the corresponding A values of 1,1×10-1,1×10-2,1×10-3,1×10-4 μg/mL concentrations of As2O3 were 0.48,0.81,0.93,0.97,0.97 respectively.Conclusion：In vitro study,As2O3 caused obvious inhibition of tumor cell proliferation,and the inhibition rates showed a dose-dependent pattern.