食管癌组织表皮生长因子受体Ⅲ型突变体表达及其意义的探讨

Expression and significance of epidermal growth factor receptor variant Ⅲ in human esophageal carcinoma tissues

目的:检测表皮生长因子受体Ⅲ型突变体(EGFRvⅢ)在食管癌组织中的表达,探讨其与食管癌发生、发展的关系。方法:采用免疫组化和蛋白质印迹实验检测EGFRvⅢ在66例人食管癌组织的表达情况,评价EGFRvⅢ表达在食管癌患者的性别、年龄分布、肿瘤大小、生长方式、TNM分期和淋巴结转移等临床病理参数的表达分布情况。结果:免疫组化结果显示EGFRvⅢ主要分布于胞膜及胞质内,在肿瘤及正常组织表达的平均净灰度值分别为22.46±4.21和5.54±3.01,两者相比差异有统计学意义,t=6.701,P=0.001。蛋白质印迹实验结果显示,EGFRvⅢ在肿瘤及瘤旁正常组织中表达的平均净灰度值分别为0.828±0.15和0.083±0.049,两者相比差异有统计学意义,t=9.362,P=0.000。两种检验方法均显示,不同性别、年龄、肿瘤大小、生长方式组间EGFRvⅢ表达,差异无统计学意义(P>0.05),TNM分期、淋巴结转移和病理分级中EGFRvⅢ表达差异均有统计学意义,P<0.05。EGFRvⅢ在两种实验方法的一致性检验(r=0.917,P<0.001),提示两种方法结果一致性好。结论:EGFRvⅢ可能参与食管癌发生,并在食管癌的侵袭和转移中起重要作用。

OBJECTIVE： To detect the expression and significance of epidermal growth factor receptor variant Ⅲ （EGFRvⅢ） in the genesis and development of human esopha geaI carcinoma. METHODS： Imrnunohistochemistry and Western-blot analysis were used to semiquantitatively detect the ex pression of EGFRvⅢ protein in 66 human esophageal carcino ma cases and the relationship between various clinicopathologic factors and EGFRv Ⅲ protein was analyzed. RESULTS： The average gray scale values of esophageal carcinoma and normal tissues by immunohistochemistry were 22.46 ± 4.21 and 5.54 ± 3.01 respectively, which showed a significant difference （t=6.701, P=0.001）. The average gray scale values between them by Western blot were 0. 828±0. 15 and 0. 083±0. 049 respectively, which showed a significant difference （t=9. 362, P〈 0. 000）. The significant differences were observed in TNM stage, lymph node metastasis and classification （P〈 0.05） , but no significant differences were found in sex, age, tumor size and growing type （P〉0.05）. The consistency analysis between immunohistochemistry and Western blot showed the consistency coefficient （r=0.917, P〈0.001）, suggesting a good consistency. CONCLUSIONS： Human esophageal carci noma tissues express EGFRvⅢ. EGFRvⅢ may play an important role in the genesis and development of human esophageal carcinoma.