摘要
目的:研究整合素αvβ6亚基αv及β6对高转移卵巢癌细胞系HO-8910PM增殖及侵袭的影响。方法:流式细胞仪检测HO-8910PM细胞αv及β6阳性表达率,MTT实验观察抗αv及抗β6单抗对HO-8910PM细胞增殖的影响,细胞侵袭实验观察抗αv及β6单抗对该细胞系侵袭的影响。结果:HO-8910PM细胞胞质内表达αvβ6,但表达率均不高,αv阳性表达率为25.7%,β6为28.3%;MTT实验显示,αv单抗可明显抑制HO-8910PM细胞增殖(P<0.05),β6单抗抑制肿瘤增殖作用不明显。细胞侵袭实验显示,β6单抗能明显抑制HO-8910PM细胞对人工基底膜胶的侵袭穿透能力(P<0.05)。结论:整合素αvβ6的αv亚基主要参与卵巢癌肿瘤细胞的增殖过程,β6亚基更多参与卵巢癌肿瘤细胞的侵袭过程。
OBJECTIVE： To investigate the influence of subunits αv and β6 of integrin αvβ6 on proliferation and invasion of ovari an cancer cell HO-8910PM. METHODS：Detect the expression of inte grin αvβ6 in cytoplasm of HO-8910PM cells with flow cytometry, and confirm the positive expression of αv and β6 in this cell line. The influence of proliferation in HO-8910PM was observed by MTT test. The influence of invation in HO-8910PM with different concertration and on different time was observed by Transwell chambers. RESULTS： Subunits αv and β6 of integrin αvβ6 were expressed in the cytoplasm of ovarian cancer cell HO-8910PM. But the expression rate is not high. The average positive expression rates of αv and β6 are 25.7% and 28.30/00. The proliferation test showed that anti-αv inhibited the cells＇ proliferation significantly （P〈0.05）. Anti-β6 inhibited the cells＇ prolif eration little. The result of Transwell system showed that anti -β6 inhibi ted the invasion in the artificial basement membrane significantly （P（ 0.05）. CONCLUSION： Subunit αv may participate more in the process of ovarian cancer cells＇ proliferation, and subunit β6 may participate more in the process of ovarian cancer ceils＇ invasion.
出处
《中华肿瘤防治杂志》
CAS
2009年第23期1844-1847,共4页
Chinese Journal of Cancer Prevention and Treatment
基金
山东省自然基金(Y2006C93)
