血清肿瘤标志及热休克蛋白60联合检测对胃癌早期诊断的价值

Combined determination of serum tumor marker and heat shock protein 60 for early gastric cancer diagnosis

目的:探讨血清肿瘤相关抗原CA199、CA242、CA724及HSP60的含量与胃癌的关系。方法:采用电化学发光法、放射免疫法及酶联免疫法分别对63例胃癌患者血清CA199、CA242、CA724及HSP60水平进行检测,并与30例慢性萎缩性胃炎伴重度不典型增生(CAGD)、30例正常对照组比较,分析其与胃癌恶性程度、转移和临床分期的关系。结果:胃癌患者血清CA199、CA242、CA724及HSP60水平均明显高于CAGD与正常对照组(P<0.01),血清CA199、CA242、CA724及HSP60的阳性率与胃癌恶性程度、转移和临床分期有关(P<0.05),4项联合检测敏感度及特异度分别可达85.6%和92.3%,4项指标联合检测可明显提高胃癌诊断的敏感度及特异度。结论:胃癌患者血清CA199、CA242、CA724及HSP60水平是胃癌发生、发展的重要因素,4项联合检测对胃癌患者诊断、治疗和预后判断等具有重要意义。

OBJECTIVE： To explore relationship between serum tumor-associated antigens CA199, CA242,CA724 and HSP60 levels and gastric cancer. METHODS： A total of 63 gastric cancer patients serum CA199,CA242,CA724 and HSP60 levels were determined by electrochemiluminescence, radioimmunoassay and ELISA, compared with 30 CAGD patients and 30 normal people as control group, the relationship between them and gastric cancer malignity degree, metastasis and clinical staging were analyzed. RESULTS：Gastric cancer patients serum CA199, CA242, CA724 and HSP60 levels were obviously higher than those of CAGD group and control group （P〈0.01）, positive rates of serum CA199,CA242, CA724 and HSP60 have relationship with gastric cancer malignity degree, metastasis and clinical staging （P〈0.05）, and four items combined determination sensitivity and specificity were 85.6% and 92.3% respectively, four items combined determination could increase sensitivity and specificity of gastric cancer diagnosis. CONCLUSION： Gastric cancer patients serum CA199, CA242, CA724 and HSP60 levels were important factor for gastric cancer genesis and development, and four items combined determination have important significance for gastric cancer diagnosis, therapy and prognosis estimation.