青藤碱影响HeLa细胞凋亡的COX-2信号通路的研究

The study of sinomenine on apoptosis in HeLa cell via COX-2 pathway

目的:研究青藤碱对宫颈癌HeLa细胞增殖的影响及其机制。方法:不同浓度青藤碱分别处理体外培养的人宫颈癌细胞系HeLa细胞,采用MTT法检测处理72h后HeLa细胞的增殖,流式细胞仪测定细胞凋亡。Westernblot检测COX-2和Bcl-2蛋白表达情况。结果:0.2、0.4mmol/L青藤碱处理HeLa细胞72h后,细胞增殖明显受到抑制;Westernblot分析表明,中高剂量组COX-2和Bcl-2蛋白表达水平下降;流式细胞仪细胞凋亡分析表明,各剂量组细胞凋亡率较对照组升高。结论:青藤碱在体外能抑制宫颈癌细胞生长,其机制可能与其抑制COX-2和Bcl-2蛋白表达,促进细胞凋亡相关。

Objective：To research sinomenine（SIN） intervention the proliferation of HeLa cell and induce apoptosis of tumour cell.Methods：HeLa cell were incubeded in vitro.This cell were divided into 3 experimental groups and 1 control group.The experimental group were given different dose SIN.The control group were given RPMI 1640.After incubated 72 hours,the proliferation of HeLa cell were detected by MTT and COX-2,Bcl-2 were investigated by Westernblot.To observe the general state of HeLa cell the Flow Cytometer were used to detect apoptosis of HeLa cell.Rseult：1.The proliferation of HeLa cell was inhibited after 72h with 0.2,0.4mmol/L SIN.2.The expression of COX-2 and Bcl-2 in middle and high dose experimental groups were decrease compare with control group（P0.05）.3.The apoptosis rate were increased in experimental groups compare with control group（P0.05）.Result：SIN can inhibit the proliferation of HeLa cell.The probably reason is SIN coult inhibit COX-2 and Bcl-2 expression.