青蒿琥酯对H22荷瘤小鼠肿瘤组织FasL的影响

Effect of artesunate on tumor FasL expression in mice of transplanted hepatocarcinoma 22

目的:观察青蒿琥酯对H22荷瘤小鼠肿瘤FasL表达的影响。方法:采用肿瘤细胞皮下注射法建立H22荷瘤小鼠模型,随机分为模型对照组、环磷酰胺组、青蒿琥酯低、高剂量组(15mg/kg、45mg/kg),造模后24小时开始腹腔注射给药,从D5开始,每二天测定肿块的长径(cm)及与之垂直的短径(cm)一次。记录结果并计算肿瘤体积,停药次日处死小鼠,完整剥离肿块,计算抑瘤率,免疫组化法检测肿瘤FasL表达。结果:与模型对照组比较,青蒿琥酯各剂量组均不同程度的抑制肿瘤生长,其中青蒿琥酯高剂量组与CTX组相当,青蒿琥酯高剂量组降低肿瘤组织FasL表达,优于模型组与CTX组。结论:青蒿琥酯明显抑制肿瘤组织生长,抑瘤机制可能与抑制肿瘤组织FasL表达有关。

Objective：To observe the inhibitory effect of artesunate（ATS） on tumor FasL expression in murine transplanted hepatocarcinoma 22（H22） in vivo and to reveal ATS tumor inhibitary mechanism.Methods：Model was H22 bearing mice by subcutaneous xenografts.The tumor bearing mice were randomly divided into model group（Model）,cyclophosphamide group（CTX） and artesunate treatment groups（ATS 15mg/kg、ATS 45 mg/kg）,10 in each group.These mice were injected intraperitoneally with normal saline,CTX 20mg/Kg or artesunate different doses（15mg/kg、45mg/kg）,qd,respectively for 15 days.Tumors long diameter A（cm） and vertical short diameter B（cm） were measured every 2 days from D5 to calculated tumor valume.The mice were killed and tumor was taken out wholely and weighted,then tumor inhibitory rate was calculated.Immunohistochemistry was used to detect FasL expression in tumors.Results：Artesunate treated groups with different dose could inhibit hepatocarcinoma growth（P0.01）.Artesunate 45 mg/kd group had the similar tumor inhibitory rate with CTX,which was better than the artesunate low dose group（P0.01）.Artesunate high dose group could depress FasL expression in tumors.The depression in artesunate was better than in model group and CTX group（P0.05）.Conclusion：Artesunate can inhibit hepatocareinoma growth,and the mechanism is concerned with depressing FasL expression in tumor.