丹参多酚酸盐对巨噬细胞抵抗素表达的影响

Research on effects of salvianolate on expression of resistin

目的:探讨丹参多酚酸盐治疗动脉粥样硬化的可能分子靶标。方法:将单核巨噬细胞U937设为对照组、模型组、药物处理组。其中,模型组、药物处理组以ox-LDL刺激1h,再于药物处理组中加入丹参多酚酸盐。待药物作用细胞3h后,RT-PCR法检测巨噬细胞Resistin、SRA、CD36mRNA表达水平。结果:与对照组相比,模型组Resistin、SRA mRNA表达水平均增高(P<0.01),药物处理组Resistin、SRA mRNA表达水平无差异(P>0.05);与模型组相比,药物处理组Resistin、SRA mRNA表达水平均降低(P<0.05)。各组细胞CD36 mRNA表达未见显著变化。且丹参多酚酸盐对抵抗素表达的影响作用与对清道夫受体A表达的影响作用呈正相关。结论:丹参多酚酸盐可能以抵抗素作为其药理作用的分子靶标之一,且对抵抗素表达的影响可能与清道夫受体A的表达受抑制有关。

Objective：To investigate the maybe molecule target which salvianolate is how to treat the artherosclerosis（AS）.Methods：The U937 monocyte-derived macrophages were separated to three groups：control group,model group and treated group.The model group and treated group were stimulated by ox-LDL.After 1h we added the salvianolate to treated group.Three hours later,we detected the mRNA expression of resistin,SRA and CD36 in macrophages by RT-PCR.Results：Compared with control group,model group significantly increased the mRNA expression of resistin and SRA（P0.01）,but treated group didn＇t change the mRNA expression of the two（P0.05）;Compared with model group,treated group significantly degraded the mRNA expression of resistin and SRA（P0.05）.There was no change in mRNA expression of CD36.And there was a positive correlation that the effects of salvianolate on expression of resistin and SRA.Conclusion：Resistin maybe one of the molecule targets in pharmacological action of salvianolate.And the effects of salvianolate on expression of Resistin is associated with the inhibited expression of SRA.