白藜芦醇联合顺铂对FRH-0201细胞的影响

Effect of resveratrol combined with DDP on FRH-0201 cholangiocarcinoma cell line

目的:探讨白藜芦醇(RES)与顺铂(DDP)联合应用对肝门部胆管癌细胞系FRH-0201的影响。方法:不同浓度的RES、DDP单独及联合作用FRH-0201细胞不同的时间,光学显微镜观察细胞形态学变化;MTT比色法和集落形成实验检测细胞增殖抑制作用;流式细胞术检测细胞周期改变及细胞凋亡率。结果:RES在5～320μmo/lL浓度范围内能够抑制FRH-0201细胞的增殖,并呈剂量-时间依赖关系,其作用24、48和72h的IC50分别为55.35、32.84和28.01μmol/L。RES+DDP联合应用的细胞抑制率和诱导凋亡率较二者单独作用显著升高(P<0.05)。结论:RES对FRH-0201细胞具有抑制作用,RES+DDP联合应用具有协同作用。

Objcetive： To investigate the effect of resveratrol （RES）combined with cisplatin （DDP）on the cholangiocarcinoma cell line FRH-0201 in vitro. Methods： FRH-0201 cells were exposed to different concentrations of RES or （and）DDP. Cell morphological changes were observed by light and fluorescence microscopy. Inhibition of cell proliferation was measured by MTT and colony-forming assay. And the flow cytometry were performed to detect the cell apoptosis rate and cell cycle. Results： Resveratrol inhibited the proliferation of FRH-0201 cells in a time-and dose-dependent manner in the concentration of 5～320 μmol/L and the IC50 values after the exposure of 24, 48, and 72 h were 55.35, 32.84, and 28.01 μmol/L respectively. RES and DDP had synergetic effect in the inhibition of FRH-0201 cells. Conclusion： RES can inhibit the growth of FRH-0201 cells and has the synergetic effect with DDP.