血管内皮生长因子-C mRNA和微血管密度与乳腺癌预后相关性关系的探讨

Relationship between vascular endothelial growth factor-C mRNA and microvessel density and prognosis in breast cancer

目的:探讨乳腺癌组织内血管内皮生长因子-C mRNA(VEGF-C mRNA)的表达及微血管密度(MVD),分析MVD和VEGF-C mRNA表达与乳腺癌患者预后的意义。方法:采用原位杂交技术观察92例乳腺浸润性导管癌(IDC)组织中VEGF-C mRNA的表达;CD105(细胞膜糖蛋白)标记乳腺癌组织中微血管并观察MVD。结果:不同组织学分级乳腺IDC组织中VEGF-C mRNA表达差异有统计学意义,P<0.05;在淋巴结转移组VEGF-C mRNA表达明显高于淋巴结未转移组,P<0.01;VEGF-C mRNA表达阳性组MVD明显高于VEGF-C mRNA表达阴性组,P<0.001;淋巴结转移组乳腺癌组织中MVD高于淋巴结未转移组,差异有统计学意义(P<0.001),VEGF-C mRNA表达阳性组(高MVD组)较VEGF-C mRNA表达阴性组(低MVD组)5年生存率低,差异有统计学意义,P<0.05。结论:VEGF-C mRNA表达与乳腺癌淋巴结转移及MVD密切相关,检测乳腺癌组织中VEGF-C mRNA表达及观察MVD可能预测乳腺癌预后。

OBJECTIVE：To study the significance of expression of vascular endothelial growth factor-C（VEGF-C mRNA） and microvessel density（MVD） in invasive breast cancer,and investigate the relationship between VEGF-C mRNA and MVD and the prognosis in breast cancer patients. METHODS：In situ hybridization was used to detect the expression of VEGF-C mRNA. CD105 was used to detect MVD in breast cancer. RESULTS：With increasing of the pathological grade,the VEGF-C mRNA expression was high （P0.05）. The VEGF-C mRNA expression in lymph node metastasis was greater than that without lymph node metastasis （P0.01）. MVD was higher in the positive expression of VEGF-C mRNA group than that in the low expressed group （P0.001）. MVD was related to the lymph node metastasis （P0.001）. The life-tables survival analysis indicated that for the overall survival （OS）,there was a statistically significant difference between the VEGF-C mRNA expression positive （high-MVD） group and the VEGF-C mRNA expression negative （low-MVD） group （P0.05）. CONCLUSIONS：VEGF-C mRNA promotes lymph vessel in breast cancer. The expression of VEGF-C mRNA and MVD might be considered to be a reference indicator for determining the prognosis in breast cancer.