蛛网膜下腔出血后下丘脑caspase－3、bax和bcl－2的表达及意义

Expression and significance of caspase-3,bax and bcl-2 in the hypothalamus of rats after experimental subarachnoid hemorrhage

目的探讨蛛网膜下腔出血后(subarachnoid hemorrhage,SAH)下丘脑在不同时间凋亡相关因子的变化。方法成年雄性Wistar大鼠,采用枕大池内新鲜自体动脉血二次注入法建立大鼠蛛网膜下腔出血模型,用Real-Time PCR技术检测凋亡分子caspase-3和bax以及抗凋亡分子bcl-2在不同时间点的变化情况,并在变化最明显的时间点用Western blot和尼氏染色的方法进一步验证此变化。结果蛛网膜下腔出血后下丘脑凋亡分子caspase-3和bax基因表达量升高且在术后第2天达到高峰,与空白对照组相比有统计学差异(P<0.05),抗凋亡分子bcl-2表达量也有所升高(P<0.05);同时在第2天3种分子蛋白表达量较空白对照组也有明显的升高趋势。结论蛛网膜下腔出血后,凋亡及抗凋亡途径均在下丘脑内起作用,并且这种作用在48h后达到高峰。由此推断蛛网膜下腔出血后部分激素分泌紊乱可能与下丘脑凋亡有关。

Objective To investigate changes of apoptosis-related molecules at various time points after subarachnoid hemorrhage （SAH） in the hypothalamus of rats. Methods SAH models were made by the double hemorrhage method. Gene expressions of caspase-3, bax, and bcl-2 at different time points after SAH were detected using real-time PCR. In addition, western blot and Nissl staining were separately employed to further confirm protein and morphological changes at 48 h after SAH. Results Gene expressions of caspase-3, bax and bcl-2 which peaked at 48 h after surgery had a significant difference compared with the control group （ P 〈 0.05 ）. Also, compared with the control group, proteins of caspase-3, bax, and bcl-2 were significantly increased in the experimental group at 48 h（ P 〈 0.05 ）. Conclusion The activated apoptotic and anti-apoptotic pathway in the hypothalamus of rats which suffered SAH may be related to the disorder of hormone secretion.