异丙酚对大鼠急性坏死性胰腺炎的干预作用

Effects of propofol on severe acute pancreatitis in rats

目的探讨异丙酚对大鼠急性坏死性胰腺炎（ANP）的干预作用及其机制。方法54只雄性SD大鼠按随机表法分为假手术组、ANP组、异丙酚组。异丙酚组在ANP制模后经阴茎背静脉持续泵输注异丙酚10mg·kg^-1·h^-1。制模后3、6、12h分批处死大鼠，取血检测血清淀粉酶、脂肪酶、一氧化氮（NO）、TNF-α、IL-6水平及超氧化物歧化酶（SOD）活性，取胰腺组织常规病理检查并评分。结果制模后6h，假手术组、ANP组和异丙酚组的血淀粉酶水平分别为（1743±370）U／L、（7745±1030）U／L和（5529±874）U／L；脂肪酶为（274．9±36．1）U／L、（1672±262）U／L和（1219±207）U／L；TNF—α为（1．110±0．276）mg／L、（3．191±0．279）mg／L和（2．361±0．281）mg／L；IL-6为（102．6±28，5）ng／L、（334．1±34．0）ng/L和（268．6±29．8）ng／L；NO为（42．2±18．1）umol／L、（120．7±22．3）umol／L和（73．6±19．3）umol／L；SOD为（120．6±20．1）U／ml、（54．1±15．3）U／ml和（85．7±17．1）U／ml；胰腺病理评分为0．333±0．408、4．417±0．665和3．500±0．707。ANP组血清淀粉酶、脂肪酶、IL-6、TNF-α、NO水平及胰腺病理评分均较假手术组明显升高（P〈0．05），而SOD活性明显降低（P〈0．05）。异丙酚组血清淀粉酶、脂肪酶、IL-6、TNF—α、NO水平及胰腺病理评分均较ANP组明显降低（P〈0．05），而SOD活性明显升高（P〈0．05）。结论异丙酚可降低ANP大鼠血清淀粉酶和脂肪酶水平，减轻胰腺组织的损害，对ANP具有一定的治疗作用。

Objective To investigate the necrotizing pancreatitis （SAP） in rats. Methods therapeutic effects and mechanisms of propofol on acute 54 male Sprague-Dawley rats were randomized into three groups with 18 rats in each group： sham-operation group, ANP group, propofol-treated group. After ANP models were induced, propofol（ 10 mg · kg^-1 · h^-1） were injected by dorsal vein of penis in the propofoltreated group. The rats in all groups were sacrificed at 3, 6, 12 hours after induction of the model. The serum amylase, lipase, nitric oxide （NO）, TNF-α, superoxide dismutase （SOD）, IL-6 were detected; the histopathological score of pancreatic tissue were evaluated. Results At 6 h, the levels of serum amylase in sham-operation group, ANP group, propofol-treated group were （1743 ± 370）U/L, （7745 ± 1030）U/L and （5529 ± 874）U/L; the levels of serum lipase were （274.9 ± 36.1 ）U/L, （1672 ± 262 ）U/L and （1219 ± 207 ） U/L; the levels of serum TNF-α were （ 1.110 ± 0. 276 ） mg/L, （ 3. 191 ± 0. 279 ） mg/L and （2. 361 ± 0. 281 ） mg/L; the levels of serum IL-6 were （102.6 ±28.5）ng/L, （334.1 ±34.0）ng/L and （268.6 ±29.8）ng/L; the levels of serum NO were （42.2 ± 18.1 ） umol/L, （ 120.7 ± 22.3 ） umol/L and （73.6 ± 19.3 ） umol/L; the levels of serum SOD were （120.6 ±20. 1）U/ml, （54.1 ±15.3）U/ml and （85.7±17. 1）U/ml; the histopathological scores were 0. 333 ± 0. 408, 4. 417 ± 0. 665 and 3. 500 ± 0. 707. The serum levels of amylase, lipase, NO, TNF-α, IL-6 and the histopathological score of pancreas in ANP group were significantly higher than those in sham-operation group group （ P 〈 0.05 ）, while the serum levels of SOD were significantly lower （P 〈 0.05）. The serum levels of amylase, lipase, NO, TNF-α, IL-6 and the histopathological score of pancreas in propofol-treated group were significantly lower than those in the ANP group （ P 〈 0.05 ） , while the serum levels of SOD were significantly higher （P 〈 0.05）. Conclusions Propofol could decrease the levels of serum amylase and lipase, reduce pancreatic tissue damage, and had therapeutic effects on ANP in rats.