部分照射离体血对淋巴细胞染色体畸变形成的影响

Effects of partial radiation in vitro on chromosome aberrations in human peripheral blood lymphocytes

目的分析^60Coγ射线部分照射离体血对人外周血淋巴细胞染色体畸变形成的影响。方法用2Gy^60Coγ射线37℃照射人外周血后以不同比例混合后进行培养、制片和分析染色体畸变。结果染色体畸变随照射血比例的增加而增加，与单纯照射组相比，混合照射血的染色体畸变率高。1：1比例混合估算出的剂量为1．27Gy，大于1Gy；0．5：1比例混合估算出的剂量为0．93Gy，大于0．5Gy；而在1：0组，照射剂量与估算剂量基本一致。结论染色体畸变可以作为估算非均匀照射的生物学指标之一。

Objective To analyze the chromosome aberrations induced by partial radiation in human peripheral blood lymphocytes in vitro. Methods Heparinized whole blood samples were exposed to 2 Gy ^60Coγ-rays at 37℃ , and then mixed with non-irradiated blood by different ratio. The slides were prepared after culturing and the unstable aberrations were analyzed. Results The chromosome aberrations had a good relationship with the ratio of irradiated blood. The chromosome aberrations in partial irradiated group were higher than that in the irradiated group. The estimated dose was 1.27 Gy when the ratio was 1：1, greater than the dose of 1 Gy. The estimated dose was 0.93 Gy when the ratio was 0.5： 1, also greater than 0.5 Gy. But when the ratio was 1 ： 0, the radiation dose was accordant with the estimated dose. Conclusions Chromosome aberrations could be a biomarker for estimating the uneven irradiation.