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RNA干扰分化抑制因子-1基因表达对食管鳞状细胞癌增殖与凋亡的影响 被引量：5

Effect of Interfering Id-1 Gene Expression by RNA on Proliferation and Apoptosis of Esophageal Squamous Cell Carcinoma
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摘要 目的研究针对分化抑制因子-1的siRNA(si-Id-1)对人食管鳞状细胞癌(ESCC)细胞增殖和凋亡影响的可能机制。方法阳离子脂质体法介导si-Id-1转染ESCC TE-1细胞株,倒置荧光显微镜下观察细胞形态学变化并计算转染率,RT-PCR检测Id-1的mRNA表达水平;免疫印迹法检测Id-1的蛋白表达水平;细胞增殖实验检测转染前后TE-1细胞增殖能力变化;流式细胞术检测各组细胞的周期变化和凋亡指数。结果si-Id-1转染前后的TE-1细胞株Id-1的mRNA和蛋白表达水平有明显差异(P<0.05);转染后的TE-1细胞较其亲代细胞增殖能力降低,凋亡程度增强(P<0.05);转染后的TE-1细胞周期停滞在G0/G1期,G1期细胞数增多,S期细胞数减少(P<0.05)。结论利用脂质体将si-Id-1转染至ESCC的TE-1细胞是切实可行的;si-Id-1可以有效沉默TE-1细胞Id-1的表达,从而抑制ESCC细胞增殖。 Objective To investigate the effect of si-Id-1 on the proliferation and apoptosis of esophageal squamous cell carcinoma. Methods Si-Id-1 was synthesized and transfected into TE-1 cells by Lipofectamine 2000. Cell morphological change and the transfection index were observed with inverted fluorescent microscope. The mRNA level of Id-1 was examined by RT-PCR and the protein level of Id-1 was examined by Western blot. Cell proliferation of TE-1 cell line was examined by CCK-8 assay after transfection. Cell cycle distribution and apoptotic index were detected by flow cytometry. Results Compared with that of the control groups, the mRNA level of Id-1 in TE-1 cell line dramatically reduced after TE-1 cell line was transfected with si-Id-1（P〈0.05）. cell proliferation was inhibited significantly after si-Id-1 transfection（P〈0.05） ; the percentage of TE-1 in G1-pbase was significantly higher than that in control groups; the percentage of TE-1 apoptosis cell was significantly higher than that in control groups （P（0.05）. Conclusion The transfection of si-Id-1 into TE-1 cell line by lipofectamine 2000 is safe, efficient and low toxic. The transfected si-Id-1 can effectively inactivate the expression of Id-t in TE-1 cell line and slow down the cell proliferation of esophageal squamous cell carcinoma.
作者 曾达武 方明霞 刘豫瑞
机构地区 福建医科大学附属第一医院肝病中心
出处 《福建医科大学学报》 2009年第6期447-451,共5页 Journal of Fujian Medical University
关键词 抑制因子 免疫 癌 鳞状细胞 食管肿瘤 RNA 小分子干扰 细胞凋亡 细胞增殖 suppressor factors, immunologic carcinoma, squamous cell esophageal neoplasms cell apoptosis cell proliferation RNA, small interfering
分类号 R735.102.2 [医药卫生—肿瘤] R392.3 [医药卫生—免疫学]
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参考文献11

  • 1Mariette C, Piessen G,Triboulet J P. Therapeutic strategies in esophageal carcinoma : role of surgery and other modalities[J]. Lancet Oncol , 2007,8(6) :544-553.
  • 2Hui C M,Cheung P Y,Ling M T, etal. Id-1 promotes proliferation of p53-deficient esophageal cancer cells[J]. Int J Cancer, 2006,119(3):508-514.
  • 3Lee T K,Poon R T,Yuen A P, etal. Regulation of angiogenesis by Id-1 through hypoxia-inducible factetor-la-mediated vascular endothelial growth factor up-regulation in hepatoeellular carcinoma[J].Clin Cancer Res , 2006,12(13) :6910-6919.
  • 4刘豫瑞,方明霞,曾达武.Id-1在肝细胞癌中的表达及其临床意义[J].福建医科大学学报,2009,43(1):32-36. 被引量：7
  • 5Bass B L. RNA intefrerence, the short answer[J]. Nature, 2001,411 (6836) :428-429.
  • 6刘豫瑞,庄则豪,李友炳,黄雄飞,曾达武.ID-1、Ki-67及Bcl-2在食管鳞状细胞癌中的表达及意义[J].中华消化内镜杂志,2005,22(5):327-330. 被引量：10
  • 7Davidson B L, Boudreau R L. RNA interference: a tool for querying nervous system function and an emerging herapy[J]. Neuron, 2007 ,53 (6) : 781-788.
  • 8Wesetrhout E M, Berkhout B. A systematic analysis of the effect of target RNA structure on RNA inetfrerence[J]. Nucleic Acids Res, 2007,35 (13):4322-4330.
  • 9Li B,Cheung P Y,Wang X, et al. Id-1 activation of PISK/ Akt/NF-kappa B signaling pathway and its significance in promoting survival of esophageal cancer cells[J]. Carcinogenesis J T- Carcinogenesis, 2007,28(11) ,2313-2320.
  • 10Swarbrick A, Akerfeldt M C, Lee C S, et al. Regulation of cyclin expression and cell cycle progression in breast epithelial cells by the helix loop helix protein Id-1[J]. Oncogene, 2005, 24(3) :381-389.

二级参考文献35

  • 1刘豫瑞,庄则豪,李友炳,黄雄飞,曾达武.ID-1、Ki-67及Bcl-2在食管鳞状细胞癌中的表达及意义[J].中华消化内镜杂志,2005,22(5):327-330. 被引量：10
  • 2Lasorella A,Uo T,lavarone A. ID proteins at the cross-road of development and cancer [J]. Oncogene, 2001, 20:8326-8333.
  • 3Lyden D, Young A Z,Zagzag D, et al. ID1 and ID3 are required for neurogenesis, angiogenesis and vascularization of tumour xenografts[J]. Nature, 1999,401 : 670-677.
  • 4Norton J D. Id helix-loop-helix proteins in cell growth, differentiation and tumorigenesis[J]. Cell Science, 2000,113 :3897- 3905.
  • 5Schindl M, Schoppmann S F, Strobel T, et al. Level of Id-1 protein expression correlates with poor differentiation, enhanced malignant potential and more aggressive clinical behavior of epithelial ovarian tumors[J]. Clin Cancer Res, 2003,9:779-785.
  • 6Lin M T,Wang X, Quyang X S, et al. Id-1 expression promotes cell survival through activation of NF-signaling pathway in prostate cancer cells[J]. Oncogene, 2003,22 :4498-4508.
  • 7Midorikawa Y,Tsutsumi S,Taniguchi H, et al. Identification of genes associated with dedifferentiation of hepatocellular carcinoma with expression profiling analysis[J]. Jpn J Cancer Res, 2002,93 :636-643.
  • 8Damdinsuren B,Nagano H,Kondo M, et al. Expression of Id proteins in human hepatoeellular carcinoma:relevance to tumor dedifferentiation[J]. Int J Oncol, 2005,26 :319-327.
  • 9Idobe Y,Murawaki Y,Kitamura Y, et al. Expression of transforming growth factor-beta 1 in hepatocellular carcinoma in comparison with the non-tumor tissue[J]. Hepatogastroenterology, 2003,50:54-59.
  • 10Bedossa P, Peltier E, Terris B, et al. Transforming growth factor-beta 1 (TGF-beta 1) and TGF-beta 1 receptors in normal, cirrhotic and neoplastic human livers[J]. Hepatology,1995,21:760-766.

共引文献14

同被引文献40

  • 1Ling MT, Wang Xv Zhang Xvet al. The multiple roles of Id-l in cancer progression[J]. Differentiation, 2006,74(9-10) : 481-7.
  • 2Meteoglu I, Meydan N, Erkus M. ld-t : regulator of EGFR and VEGF and potential target for colorectal cancer therapy[J]. J Exp Clin Cancer Res,2008 ,27( 1) :69.
  • 3Wilson JW,Deed RW,InoueT,et al. Expression of Id elix-loop-helix proteins in colorectal adenocarcinoma correlates with p53 expression and mitotic index[J]. Cancer Res,2001 ,61 (24) :8803-10.
  • 4Li W .Zhang CH, Hong YL.et al. Inhibitor of DNA-binding-l/ inhibitor of differentiation-l (!(}.1) is implicated in various aspects of gastric cancer cell biology[J]. Mol Bioi Rep, 2011 ,39 (3) :3009-15.
  • 5Yokota v , Mori S. Role of Id family proteins in growth control [J]. J Cell Physiol,2002, 190(1) :21-8.
  • 6Perk J .Iavarone A,Benezra R. Id family of helix-loop-helix proteins in cancer[J]. Nat Rev Cancer,200S,S(8) :603-14.
  • 7Nishiyama, Koichi MD, Takaji , et al. Idl gene transfer confers angiogenic property on fully differentiated endothelial cells and contributes to therapeutic angiogenesis[J]. Circulation, 2005, 112(18) :2840-50.
  • 8Ling MT,Wang X,Zhang X,et al.The multiple roles of Id-1 in cancer progression[J].Differentiation,2006,74(9-10):481-487.
  • 9Meteoglu I,Meydan N,Erkus M.Id-1:regulator of EGFR and VEGF and potential target for colorectal cancer therapy[J].J Exp Clin Cancer Res,2008,27:69.
  • 10Langlands K,Down GA,Kealey T.Id proteins are dynamically expressed in normal epidermis and dysregulated in squamous cell carcinoma[J].Cancer Res,2000,60(21):5929-5933.

引证文献5

二级引证文献12

福建医科大学学报
2009年 第6期

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