水通道蛋白0,1在STZ-糖性白内障发病机制中的研究

Role of Aquaporin 0 and Aquaporin 1 in the Pathogenesis of Streptozotocin-Induced Diabetic Cataract

目的研究水通道蛋白0,1(AQP0、AQP1)在链脲佐菌素(STZ)-糖尿病性白内障发病机制中的作用。方法将40只SD大鼠随机分为对照组与糖尿病性白内障组2组,用STZ腹腔注射诱发糖尿病性白内障,每周观察晶状体的变化。分别于实验开始后2,8周末摘取眼球,采用免疫组织化学染色技术检测AQP0及AQP1在晶状体中的表达,各检测10只大鼠20眼。结果对照组晶状体保持透明,糖尿病性白内障晶状体在2周末出现空泡,8周末完全混浊。AQP0及AQP1在对照组中阳性表达,糖尿病性白内障组2周表达较对照组强,组间差别具有统计学意义(AQP0:t2周末=3.598,P=0.001;AQP1:t2周末=3.103,P=0.004);糖尿病性白内障组8周表达较对照组弱,组间差别具有统计学意义(AQP0:t8周末=6.994,P<0.001;AQP1:t8周末=4.475,P<0.001)。结论AQP0及AQP1表达异常与糖尿病性白内障的发生、发展有关。

Objective To investigate the role of aquaporin 0 and 1 （AQP0, AQP1） in the pathogenesis of streptozotocin （STZ）-induced diabetic cataract. Methods Forty SD rats were randomly divided into two groups ： the normal control group and the STZ-induced diabetic cataract group. Diabetic cataact was induced by intraperitoneal injection of STZ. Lenses were examined once a week with a slit lamp. Eyes were isolated respectively at the end of 2 and 8 weeks after STZ injection （20 eyes from I0 rats per group at one time point）. The expression of AQP0 and AQP1 in both normal control lenses and diabetic cataract lenses was detected by immunohistochemistry. Results Lenses in the control group maintained transparency throughout the experimental period. Those in the STZ-induced diabetic cataract group exhibited vacuoles at the end of 2 weeks and complete opacity at the end of 8 weeks. AQP0 and AQP1 in the normal control group were positively expressed. At the end of two weeks, the expression of AQP0 and AQP1 in the STZ-induced diabetic cataract group was signifieantly stronger than that in the normal control group （AQP0：t2=3. 598,P=0. 001;AQP1：t2=3. 103,P=0. 004）. At the end of 8 weeks, the expression of AQP0 and AQP1 in the STZ-induced diabetic cataract group was significantly weaker than that in the normal control group（AQP0：t8 =6. 994, P〈0.001;AQPI：t8=4.475, P〈0. 001）. Conclusion The abnormal expression of AQP0 and AQP1 may be related to the occurrence and development of diabetic cataract.