膀胱移行细胞癌组织中p53、VEGF、PCNA的表达及临床意义

The Expression and Clinical Significance of p53,VEGF and PCNA in Bladder Transitional Cell Carcinoma Tissue

目的研究p53、VEGF、PCNA在膀胱移行细胞癌组织中的表达及与膀胱移行细胞癌病理分级、临床分期的关系。方法采用免疫组织化学SP方法检测43例膀胱移行细胞癌组织及10例正常膀胱黏膜组织中p53、VEGF、PCNA的表达。病理分级按WHO标准:G1级12例,G2级24例,G3级7例。临床分期按TNM标准,分为浅表型(Tis～T1)34例,浸润型(T2～T4)9例。结果膀胱移行细胞癌组织中p53、VEGF、PCNA阳性表达率分别为48.84%、67.44%、62.79%。正常膀胱黏膜组织中p53、VEGF、PCNA均无阳性表达。p53、VEGF与膀胱移行细胞癌病理分级、临床分期有明显相关性(P<0.05);PCNA与膀胱移行细胞癌病理分级有明显相关性(P<0.05),但与临床分期无相关性(P>0.05)。结论p53、VEGF、PCNA与膀胱移行细胞癌病理分级、临床分期、预后密切相关。p53、VEGF可以作为判断膀胱移行细胞癌恶性程度和预后的重要指标,PCNA可以作为判断膀胱移行细胞癌恶性程度和预后的辅助指标。

Objective To study the expression of p53, vascular endothelial growth factor （VEGF） and proliferating cell nuclear antigen （PCNA） in bladder transitional cell carcinoma （BTCC） tissue and their relations with the pathological grades and clinical stages. Methods The expression of p53, VEGF and PCNA in 43 cases of BTCC tissue and 10 normal cases with bladder issues were determined by immunochemical SP methods. Results The positive expression rates of p53, VEGF, PCNA in BTCC were 48.84%, 67.44%, 62.79%. the negative expression of p53, VEGF, PCNA in normal cases with bladder issues. The significantly correlation was noted between p53, VEGF and the pathological grades, clinical stages of BTCC（P〈0.05）. The significantly correlation was noted between PCNA and the pathological grades of BTCC（P〈0.05）, but PCNA was not related to the clinical stages（P〉0.05）. Conclusions p53, VEGF, PCNA are closely related to the pathological grades and clinical stages of BTCC. p53, VEGF may serve as an important index to estimate the pathological grades, clinical stages and prognosis of BTCC. PCNA may serve as an auxiliary marker to estimate the pathological grades, clinical stages and prognosis of BTCC.