摘要
运用差异显示PCR(DD-PCR)方法分析棉铃虫滞育解除蛹差异表达的基因,结果得到了56个差异片段.通过RT-PCR和Real-time PCR的方法,鉴定了滞育解除过程中有3个基因表达量在下调,有4个基因表达量在上调.这7个差异表达基因中有3个和已知的基因有较高的同源性:FKBP12,esr16和NADH dehydrogenase 1 alpha subcomplex subunit 6.这些差异基因为今后研究滞育解除的分子机制提供了新的线索.
By means of mRNA differential display PCR（DD-PCR）,56 cDNA fragments have been identified from the Helicoverpa armigeras brain that are expressed differentially between the diapausing pupae and the pupae of diapause termination by injecting 20-hydroxyecdysone.RT-PCR and Real-time PCR analysis revealed that mRNA levels of the FK506-binding protein 12（FKBP12）and two unknown genes were higher in the diapausing pupae,whereas the ecdysteroid-regulated 16 kDa protein（esr16）,NADH dehydrogenase 1 alpha subcomplex subunit 6,and two unknown genes were higher in the pupae of diapause termination.These results provide new clues to researches on the molecular mechanism of diapause.
基金
国家自然科学基金(30730014)资助
关键词
滞育
20-羟基蜕皮酮
差异显示PCR
棉铃虫
diapause
20-hydroxyecdysone
differential display PCR
Helicoverpa armigera
