宫内感染对新生仔鼠脑内蛋白GAP-43和MAP-2影响的实验研究

Effect of Intrauterine Infection on Expression Levels of GAP-43 and MAP-2 in Brain of Neonatal Rats

目的:通过观察神经生长相关蛋白(GAP-43)和微管相关蛋白-2(MAP-2)的变化,以研究宫内感染对新生儿脑发育的影响。方法:取孕17d的大鼠36只,随机取30只腹腔注射脂多糖(LPS)450μg/kg,连续注射2d,造成宫内感染模型,22d后出生的仔鼠22只作为I组;另6只注射等剂量生理盐水,将其所生仔鼠作为对照组(N组)。2组均于生后即刻(0日龄)、14和28日龄3个时间点观察仔鼠脑内GAP-43和MAP-2的表达情况。结果:0日龄I组仔鼠脑皮层、海马及内囊GAP-43、MAP-2免疫阳性面积比(AF)较对照组明显减少(P<0.05)。结论:GAP-43和MAP-2是神经生长发育和损伤修复的重要标记物之一,宫内感染可干扰仔鼠脑组织GAP-43和MAP-2的表达。

Objective：To study the effects of intrauterine infection on the brain development in infants by observing the expression levels of growth associated protein-43 （GAP-43） and microtubule associated protein-2 （MAP-2）. Methods： Thirty pregnant rats were consecutively injected with LPS （450 μg/kg） on the gestation 17 day and 18 day, to establish a model of intrauterine infection; the same volume of normal saline was injected into 6 pregnant rats as normal group （group N）. Twenty-two neonatal rats from LPS group served as injury group （group I）. The expression levels of GAP-43 and MAP-2 in brain of neonatal rats of the two groups were detected at the 0th, 14th, and 28th day respectively. Results： As compared with group N, the immunoreactive positive area ratio （AF） of GAP-43 and MAP-2 in brain （cortex, hippocampus, internal capsule） of neonatal rats of the group I at the 0th day was significantly reduced （P〈0.05）. Conclusion： Intrauterine infection inhibits the brain development in neonates.