摘要
目的:探讨X线修复交叉互补组1基因(X-ray repair cross complementing group 1,XRCC1)、人类着色性干皮病基因D(xeroderma pigmentosum complementary group D,XPD)多态性与喉癌遗传易感性的关系。方法:采用病例-对照设计方法对72例经病理确诊的喉鳞状细胞癌患者(病理组)和随机抽取的72例非癌症患者为对照组,均进行一般资料问卷调查和抽取外周静脉血进行XRCC1-Arg399Gln、XPD-Lys751Gln多态性检测和聚合酶链反应-限制性片段长度多态性分析法(PCR-RFLP)检测。结果:与携带XRCC1-399野生型(Arg/Arg)个体相比,病例组XRCC1第399位密码子杂合型(Arg/Gln)及突变型(Gln/Gln)分布频率高于对照组(P<0.05),携带该基因型的个体喉癌的发病风险升高3.37(OR=3.37,95%,CI=1.69-6.70)倍;XPD-Lys751Gln各基因型差异2组间无统计学意义;交互作用分析显示,吸烟与不吸烟患者比较,携带XRCC1-399Arg/Gln+Gln/Gln基因型个体的喉癌发病风险差异未发现有统计学意义(χH2=0.09)。结论:XRCC1-399位点Arg→Gln的氨基酸替换可能导致喉癌的发病风险增加,XRCC1-Arg399Gln多态性可能与喉癌的遗传易感性有关。
Objective：To evaluate the association between DNA repair gene （XRCC1, XPD） polymorphisms and the risk of laryngeal carcinoma. Methods： A case-control study was performed on 72 patients with laryngeal squamous carcinoma and 72 random controls with no laryngeal carcinoma and no inherited disease. The PCR-RFLP method was used to analyze the XRCC1-Arg399Gln, XPD-Lys751Gln polymorphisms. Results： The frequency of XRCC1-399Arg/Gln＋Gln/Gln genotypes in the case group was higher than in the control group （P〈0.05）. There was a 3.37-fold （OR=3.37, 95% CI=1.69-6.70） increased risk of laryngeal carcinoma for individuals carrying XRCC1-399Arg/Gln＋Gln/Gln genotypes, compared with subjects carrying XRCC1-Arg/Arg genotype. There was no significant difference in XPD-Lys751Gln polymorphisms between two groups. No statistically significant difference was found between the smoking group and non-smoking group in risk of laryngeal carcinoma of XRCC1-399Arg/Gln＋Gln/Gln genotypes. Conclusion： The amino acid replacement of XRCC1-399Arg→Gln might lead to an increased risk of laryngeal carcinoma. Our study indicated a possible association between XRCC1 gene polymorphisms and laryngeal carcinoma.
出处
《中国康复》
2010年第1期9-12,共4页
Chinese Journal of Rehabilitation
基金
湖南省教育厅科研基金(N.07C061)
