几种纤维素酶制剂水解和吸附性能的研究

Study on Enzymatic Hydrolysis and Adsorption Properties of Several Cellulases

比较了商品纤维素酶和自产纤维素酶在蛋白组分及蛋白组分含量上存在的差异。商品纤维素酶水解稀酸预处理和蒸汽爆破预处理的玉米秸秆,其水解得率均低于自产纤维素酶。以蒸汽爆破的玉米秸秆为碳源制备纤维素酶,添加外源8 IU/g(以纤维素计)的β-葡萄糖苷酶,水解蒸汽爆破的玉米秸秆48 h,纤维素水解得率为90.08%;水解液中纤维二糖的质量浓度从17.06 g/L降低到1.12 g/L,相应葡萄糖质量浓度从21.09 g/L提高到44.01 g/L,可发酵性糖从55.28%提高到97.52%。微晶纤维素对商品酶和自产酶的吸附在30 m in达到平衡,且符合Langmu ir等温吸附方程;由Langmu ir常数分析得知两类酶均来自里氏木霉,且对微晶纤维素的亲和力相差不大。

The differences of proteins between cellulases from Trichoderrna reesei Rut C30 produced in this laboratory and commercial eellulases were evaluated. All three cellulase preparations produced in this laboratory showed better performance than commericial cellulases on hydrolysis of steam-exploded and dilute-acid-pretreated corn stover. The cellulase, produced from steamexploded corn stover, could obtain hydrolysis yield of 90.08 % on steam-exploded corn stover through supplementing/3-glueosidase of 8 IU/g cellulose; the content of cellobiose was decreased from 17.06 to 1.12 g/L; the content of glucose was increased from 21.09 to 44.01 g/L and the ratio of fermentable sugar was enhanced from 55.28 % to 97.52 %. The adsorption of eellulases protein on microerystalline cellulose reached equilibrium in 30 min. From the Langmuir adsorption isotherm, all cellulases had similar affinity for microcrystalline cellulose and maybe derived from Trichoderrna reesei.