心房利尿钠肽和脑利尿钠肽基因多态性与原发性高血压病的关联性研究

Association of Polymorphisms in Atrial and Brain Natriuretic Peptide Gene with Essential Hypertension

目的探讨心房利尿钠肽(ANP)基因位点rs5065和脑利尿钠肽(BNP)基因位点rs198389基因多态性与原发性高血压病(EH)的关系。方法采取病例对照方法设计,收集EH患者976例和与之年龄、性别频率匹配的非高血压(NT)对照976例,应用实时荧光定量多聚酶链反应(real-time Q-PCR)技术之TaqMan探针法检测了ANP基因rs5065和BNP基因rs198389的多态性基因型。结果BNP基因位点rs198389基因型AA和AG+GG频率分布、A等位基因和G等位基因频率分布在EH组和NT组之间差异具有显著性意义(P<0.05),AG+GG基因型和G等位基因频率分布在EH组(30.5%和17.3%)明显高于NT组(26.1%和14.1%)(均P<0.05)。ANP基因位点rs5065基因型分布在两组人群中未发现明显差异(P>0.05)。对rs198389采用非条件Logistic回归分析,与基因型为AA者相比,在未调整其它危险因素时,AG+GG基因型使其患EH的危险性增加(P=0.04),调整其它危险因素后,得到了同样的结果(P=0.02)。与A等位基因相比,G等位基因使患EH的危险性显著增加(P=0.04)。在rs5065非条件Logistic回归分析中,调整其它因素前与后均未见明显差异(均P>0.05)。结论BNP基因位点rs198389基因多态性与EH的发病危险性显著相关。ANP基因位点rs5065基因多态性与EH的发病危险性无显著相关性。

Objective To investigate the associations of atrial natriuretic peptide（ANP）rs5065 and brain natriuretic peptide （BNP）rs198389 gene polymorphisms with human essential hypertension（ EH ）. Methods A total of 976 patients with EH and 976 age-and sex-frequency matched normotensive（NT）control subjects were collected. The ANP rs5065 and BNP rs198389 potymorphisms were genotyped by real time quantitative polymerase chain reaction （real-time Q-PCR）method Taqman probe. Results There was significant difference in frequency distribution of AA and AG＋GG genotypes, A and G allele of BNP rs198389 between the EH and NT groups（P〈0.05）. The frequency of AG＋GG genotype and G allele was significantly higher in the EH group（30.5% and 17.3%）than in the NT group（26.1% and 14. 1%）. There was no difference in distribution of ANP rs5065 polymorphism between EH and NT groups（P〉0. 05）. By unconditional Logistic regression analysis of rs198389, before adjusted for the E H risk factors, AG＋GG genotype presented a significantly higher risk of EH （P= 0.04）than AA genotype,and this risk was still significant after adjusted for the EH risk factors（P：0.02）. G allele had more significantly increased risk of EH than A allele（P=0.04）. However,there was no significant difference before or after adjusted for the EH risk factors in unconditional Logistic regression analysis of rs198389. Conclusion rs198389 polymorphisms of BNP gene may be associated with EH,but rs5065 polymorphisms of ANP gene may be not associated with EH.