重组白细胞介素23抗小鼠系统性白假丝酵母菌感染的研究

Effect of Recombinant Interleukin-23 on Systemic Candidasis in Mice

目的探讨重组白介素23在抗小鼠系统性白假丝酵母菌感染中的作用。方法建立环磷酰胺诱导的免疫抑制小鼠系统性白假丝酵母菌感染动物模型,设置对照组(单纯白假丝酵母菌感染组)与处理组(白假丝酵母菌感染前注射重组白介素23)。用平皿稀释法检测肾脏、脾脏组织菌落形成单位(CFU)数目;制作肾、脾脏组织病理学标本,评估其病理学分级;并通过酶联免疫吸附试验(ELISA)检测脾脏干扰素γ分泌水平。结果感染后2、3、7 d,处理组肾脏CFU值明显低于对照组(均P<0.01);处理组脾脏组织CFU值也低于对照组,但差异无统计学意义(P>0.05)。肾脏组织病理学评分处理组低于对照组,处理组较对照组感染程度减轻,差异有统计学意义(P<0.01);脾脏组织病理学评分处理组也低于对照组,但差异无统计学意义(P>0.05)。同时检测脾脏干扰素γ分泌水平处理组明显高于对照组,差异具有统计学意义(P<0.01)。结论重组白介素23在小鼠系统性白假丝酵母菌感染中具有保护作用。

Objective To investigate the effect of recombinant murine interleukin-23 （rIL-23）on systemic candidiasis in a murine model. Methods A cyclophosphamide-induced immunosuppressed murine model of systemic candidiasis was established. The mice were divided into control group and rIL-23 treatment group. Colony forming units（CFU）of the kidney and spleen were determined by using plating dilution method. The histopathological changes and degree of infection of the kidney and spleen were graded. Meanwhile, the levels of interferon gamma（IFN-γ）in the spleen were measured by enzyme-linked immunosorbent assay. Results On the 2nd,3rd and 7th day after Candida albicans infection the number of CFU of the fungi in the kidney in the control group was significantly greater than that in rIL-23 treatment group（P〈0.01）. The number of CFU of the fungi on the 2nd, 3rd and 7th day after Candida albicans infection in the spleen in control group was also greater than that in rlL-23 treatment group,but without statistically significant difference（P〉0.05）. The scores of histopathological changes in the kidney in rIL-23 treatment group were lower than those in control group（P〈0.01）, and the degree of infection was milder in rIL-23 treatment group. The scores of histopathological changes in the spleen in rIL 23 treatment group were also lower than those in control group,but without statistically significant difference（P〉0.05）. The levels of IFN-γ in the spleen on the 2nd, 3rd and 7th day after infection in rIL-23 treatment group were significantly higher than those in control group （P〈 0.01）. Conclusion rIL-23 has protective effect on murine systemic candidiasis.