摘要
目的研究血红素氧化酶-1(HO-1)在姜黄素拮抗乙醇氧化损伤过程中所起的作用。方法通过亚细胞分离法提取微粒体,测定姜黄素预作用大鼠原代肝细胞中HO-1的活性,确定姜黄素对HO-1的诱导情况。通过抑制剂ZnPPⅨ和诱导剂Hemin的加入,抑制和诱导HO-1的表达与活性,通过检测细胞培养上清液中谷草转氨酶(AST)、乳酸脱氢酶(LDH)活性以及肝细胞的氧化-抗氧化指标,确定HO-1在姜黄素拮抗乙醇所致肝细胞氧化损伤过程中的作用。结果姜黄素能够诱导HO-1的活性,在姜黄素15μmol/L和提前1 h作用时HO-1水平达到高峰。且HO-1的活性和表达与肝细胞的抗氧化水平明显相关。结论姜黄素对肝细胞有明显的保护作用,主要表现在提高细胞HO-1的活性及表达水平。
Abstract Objective To investigate the role of heine oxygenase-1（HO-1）in the protective actions of eureumin against eth anol-induced oxidative damage. Methods Mierosomal HO-1 enzyme activities were determined in rat primary hepatocytes pre treated by curcumin. AST, LDH release and hepatic oxidative/antioxidant status were measured with or without ZnPPⅨ/Heroin as a classic HO-1 inhibitor/inducer, respectively. Results Curcumin could induce the HO4 expression and enzyme activity, which was correlated with antioxidant levels in hepatoeytes. HO-1 induction reached a peak under administration of 15μmol/L curcumin for 1 h. Conclusion HO-1 induction by curcumin is contributed to the heptoprotective effects against ethanol-induced oxidative damage.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2010年第1期82-86,共5页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
基金
教育部新世纪优秀人才支持计划项目(No.NECT-04-0707)
