哮喘儿童红细胞补体受体1基因多态性及红细胞免疫功能的研究

Relationship between the erythrocyte CR1 genomic density polymorphism and erythrocyte immune function in children with asthma

目的研究哮喘儿童红细胞补体受体1（CR1）基因多态性与红细胞免疫功能的相关性。方法利用限制性内切酶HindIH、聚合酶链反应（PCR）测定65例哮喘儿童（哮喘组）和28例健康儿童（对照组）红细胞CR1基因多态性，采用红细胞C3b受体花环率（RBC—c3bRR）和红细胞免疫复合物花环率（RBC．ICRR）测定两组儿童的红细胞免疫功能，并进行比较。结果哮喘组CR1基因高表达基因（HH）、中表达基因（HL）和低表达基因（LL）型分布频率分别为43．08％（28／65）、36．92％（24／65）和20．00％（13，65），对照组分别为78．57％（22，28）、17．86％（5／28）和3．57％（1／28），两组CR1基因型的分布频率比较差异有统计学意义（P〈0．01）。哮喘组RBC—c，bRR明显低于对照组（P〈0．01），RBC．ICRR明显高于对照组（P〈0．01）。哮喘组中，HH型的RBC-C3bRR明显高于HL型，HL型的RBC．c3bRR又明显高于LL型（P值均〈0．01）；HH型的RBC—ICRR明显低于HL型，HL型的RBC—ICRR又明显低于LL型（P值均〈0．01）。结论哮喘儿童红细胞CR1基因多态性分布异常，红细胞免疫功能低下，哮喘儿童红细胞免疫功能低下程度与红细胞CR1基因多态性有关。

Objective To explore the hereditary susceptibility of children with asthma through studying the relationship between erythrocyte CR1 genomic density polymorphism and erythrocyte immune function. Methods The rates of RBC-C3bRR and RBC-ICRR were detected to the asthma group consisted of 65 children with asthma and the control group consisted of 28 normal children. The CR1 activity and genomic density polymorphism of erythrocyte from the two groups were detected by Hind Ⅲ restriction enzyme digestion, polymerase chain reaction restriction fragment length polymorphism. Results Frequencies of high expression gene （HH）, mid expression gene （HL） and low expression gene （LL） genotypes were 43.08% （28/65）, 36.92% （24/65） and 20.00% （13/65） in asthma group, and 78.57% （22/28）, 17.86% （5/28） and 3.57%（ 1/28 ） in control group respectively. A significant difference was found in the distribution frequency of CR 1 genotype between the two groups （P 〈 0.01 ）.The rates of RBC-C3b RR were significant lower and the rates of RBC-ICRR were significant higher in asthma group than those in control group （P 〈 0.01 ）. The rates of RBC-C3bRR in HH, HL and LL were decreased in order （P 〈 0.01 ） ,while the rates of RBC- ICRR in HH,HL and LL were increased in order （P 〈 0.01 ）. Conclusion It suggests that CR1 gene polymorphism may play an important role in determining susceptibility to asthma.