拟南芥转录因子CBF1和CBF4基因的克隆与序列分析

Cloning and Sequences Analysis of Transcription Factor CBF1 and CBF4 Gene in Arabidopsis thaliana

利用聚合酶链式反应技术(PCR)从拟南芥(Arabidopsis thaliana)植株中扩增出CBF1和CBF4基因,并将其连接到pGEM-Tvector载体上。PCR和酶切鉴定后进行基因测序,结果表明所克隆的CBF1和CBF4基因序列与GenBank中基因序列同源性分别可达99.84%和99.41%,说明这两个片段确为拟南芥CBF1和CBF4基因。

CBF1 and CBF4 genes were obtained by PCR technique from the plants of Arabidopsis thaliana and then connected those into the pGEM-T vector. PCR, Restriction enzyme digestion and Sequencing results showed that the cloned gene sequences of CBF1 and CBF4 have highly homologous with GenBank,reached 99.84% ,99.41% Respectively. Thus,the two fragments were proved as CBF1 and CBF4 gene fragments .