摘要
旨在探讨肺表面活性物质相关蛋白A(SP-A)在复发性外阴阴道假丝酵母菌病(RVVC)患者阴道分泌物中的表达及意义。收集RVVC患者、外阴阴道假丝酵母菌患者(VVC)及健康体检者(对照组)阴道分泌物各20例,用酶联免疫吸附测定法(ELISA)测定各组中SP-A蛋白的含量,用逆转录聚合酶链式反应(RT-PCR)方法检测各组中SP-A的基因表达情况。用ELISA方法检测各组中SP-A蛋白的含量发现,VVC组的SP-A明显高于对照组(P<0.05),RVVC组的SP-A高于对照组及VVC组(P<0.05)。RT-PCR检测结果发现,VVC组的SP-A mRNA的表达水平高于对照组(P<0.05),RVVC组的SP-AmR-NA的表达水平明显高于对照组及VVC组(P<0.05)。SP-A可能是RVVC患者抗假丝酵母菌的重要物质,其性质或数量的改变可能在RVVC的发病机制中发挥重要作用。
It was to study the expression and signification of pulmonary surfactant-associated protein-A in the vaginal fluid of recurrent vulvovaginal candidiasis(RVVC). Human vaginal fluid from 20 RVVC patients,20 VVC patients and 20 healthy women( the control group)were collected,respectively. Enzyme-linked immunosorbent assay (ELISA)was applied to detect the levels of SP-A. The expression of SP-A gene was detected by reverse transcription polymerase chain reaction (RT-PCR). Results indicated that the levels and of SP-A of VVC group were higher than that of the control group( P 〈 0.05 ). The levels of SP-A of recurrent vulvovaginal candidiasis(RVVC) group were higher than those of VVC group and control group,and the expression of SP-A mRNA in VVC was higher than that in the control group. The expression of SP-A mRNA in RVVC was significantly higher than those of VVC group and control group. Therefore,SP-A might be an essential component in RVVC resistance. The qualitative and quantitative alterations of normal SP-A may play a role in the pathogenesis of RVVC.
出处
《生物技术通报》
CAS
CSCD
北大核心
2010年第2期145-148,共4页
Biotechnology Bulletin