恶唑酰草胺及其代谢物的残留分析方法

Residues Analytical Methods for Metamifop and Its Metabolites

建立了能同时测定水稻及环境中恶唑酰草胺及其3种代谢物残留量的高效液相色谱（HPLC）法。以乙腈和水的混合液作为提取溶剂，硅胶层析柱净化，采用高效液相色谱法以乙腈-水作为流动相，梯度洗脱，紫外检测器224nm检测。结果表明：恶唑酰草胺及其3种代谢物的线性范围为0．5～20．0mg／L，相关系数达0．9993～0．9999；4种化合物在土壤、稻秆、糙米和稻壳中的最低检测浓度均为0．10mg／kg，在稻田水中的最低检测浓度为0．02mg／kg，平均回收率均为78．2％～106．9％，变异系数为2．0％～11．7％，符合农药残留测定要求。

The high performance liquid chromatographic method was developed for the simultaneous determination of metamifop and three major metabolites in rice and environment. Residues were extracted with a mixture of acetonitrile and water, followed by cleanup with a silica gel column and determined by HPLC with a linear gradient elution program of acetonitrile and water as the mobile phase and ultraviolet detection at 224 nm. The results showed that the linear range of metamifop and its metabolites were from 0.5 mg/L to 20 mg/L, with the correlation coefficients being 0.999 3 to 0. 999 9. The minimum detectable concentration of the 4 analytes in soil, rice straw, rice hulls and unpolished grain samples were all 0.10 mg/kg and 0.02 mg/kg in paddy water samples, respectively. Average recoveries were 78.2% to 106.9% , with the coefficients of variation ranging from 2.0% to 11. 7%. The results meet the requirement of pesticide residue analysis.