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SLP-2荧光表达载体的构建及其在乳腺癌细胞MCF-7中的表达与定位 被引量：1

Construction of pEGFP-C3-SLP-2 Plasmid and its Expression and Localization in MCF-7 Ceils
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摘要 人的SLP-2基因是一个新的肿瘤相关基因,它在多个癌组织里高表达,如食管鳞状细胞癌组织和肺癌组织。其中在乳腺癌组织的高表达和病人的存活率负相关,这说明该基因很可能在乳腺癌的发生中发挥重要作用。为了研究SLP-2基因在肿瘤里的功能,将人类SLP-2基因全长编码区定向连入pEGFP-C3质粒,使SLP-2蛋白可以与绿色荧光蛋白在乳腺癌细胞MCF-7内融合表达。而且细胞荧光实验发现SLP-2蛋白主要定位在MCF-7细胞的胞质内,这为进一步研究SLP-2基因在乳腺癌中的功能奠定了实验基础。 Human stomatin like protein 2 （ SLP-2 ） is a novel cancer-related gene. Its protein is overexpressed in many human cancers, such as esophageal squamous cell carcinoma and lung cancer. In Breast cancer, high-level SLP-2 expression were associated with significantly decreased survival. To study the function of SLP-2 in these cancers, the coding region of human SLP-2 gene was successfully subcloned into the eukaryotic green fluorescent protein（GFP） expressing recombinant plasmids, pEGFP-C3. The vector was then transfected into MCF-7 cells for efficient expression. Moreover, results from fluorescent observation shows that GFP-SLP-2 mainly localized in cytoplasma, thus providing a useful clue for further research on the function of SLP-2 in breast cancer.
作者 王义军 吴文韬 徐甜 向双林 张健 韩梅
机构地区 湖南师范大学生命科学学院
出处 《激光生物学报》 CAS CSCD 2010年第1期10-13,24,共5页 Acta Laser Biology Sinica
基金 国家973资助项目(2006CB943506) 长沙市科技厅计划项目(K071012-11)
关键词 SLP-2 重组质粒 融合蛋白表达 细胞定位 SLP-2 recombinant plasmid fusion protein expression cell localization
分类号 Q78 [生物学—分子生物学]
  • 相关文献

参考文献9

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共引文献27

同被引文献10

  • 1张立勇,丁芳,刘仲敏,李文东,刘芝华,李衍达.SLP-2基因反义核酸对食管鳞癌细胞系TE12生长和增殖的影响[J].癌症,2005,24(2):155-159. 被引量：24
  • 2Sandrine Da Cruz,Philippe A. Parone,Philippe Gonzalo,Willy V. Bienvenut,Daniel Tondera,Alexis Jourdain,Manfredo Quadroni,Jean-Claude Martinou.SLP-2 interacts with prohibitins in the mitochondrial inner membrane and contributes to their stability[J]. BBA - Molecular Cell Research . 2008 (5)
  • 3Wang, Yingjian,Morrow, Jon S.Identification and characterization of human SLP-2, a novel homologue of stomatin (band 7.2b) present in erythrocytes and other tissues. Journal of Biological Chemistry . 2000
  • 4Tondera Daniel,Grandemange Stéphanie,Jourdain Alexis,Karbowski Mariusz,Mattenberger Yves,Herzig Sébastien,Da Cruz Sandrine,Clerc Pascaline,Raschke Ines,Merkwirth Carsten,Ehses Sarah,Krause Frank,Chan David C,Alexander Christiane,Bauer Chri.SLP-2 is required for stress-induced mitochondrial hyperfusion. EMBO Journal . 2009
  • 5Owczarek C M,Treutlein H R,Portbury K J,Gulluyan L M,Kola I,Hertzog P J.A novel member of the STOMATIN/EPB72/mec-2 family, stomatin-like 2 (STOML2), is ubiquitously expressed and localizes to HSA chromosome 9p13.1. Cytogenetics and Cell Genetics . 2001
  • 6Kirchhof MG,Chau LA,Lemke CDet al.Modulation of Tcell activation by stomatin-like protein2. J Immunol . 2008
  • 7Wang Yueqi,Cao Wenfeng,Yu Zaicheng,Liu Zhihua.Downregulation of a mitochondria associated protein SLP-2 inhibits tumor cell motility, proliferation and enhances cell sensitivity to chemotherapeutic reagents. Cancer biology & therapy . 2009
  • 8Hájek, Petr,Chomyn, Anne,Attardi, Giuseppe.Identification of a novel mitochondrial complex containing mitofusin 2 and stomatin-like protein. Journal of Biological Chemistry . 2007
  • 9Liu Jun,Deyoung Stephanie M,Zhang Mei,Dold Lisa H,Saltiel Alan R.The stomatin/prohibitin/flotillin/HflK/C domain of flotillin-1 contains distinct sequences that direct plasma membrane localization and protein interactions in 3T3-L1 adipocytes. Journal of Biological Chemistry . 2005
  • 10曹文枫,张立勇,张斌,王跃琦,刘芝华,孙保存.SLP-2蛋白在食管鳞状细胞癌中的表达及其与肿瘤侵袭转移的关系[J].中华肿瘤杂志,2010,32(11):830-833. 被引量：14

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激光生物学报
2010年 第1期

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